目的:探讨藿香正气液(HXZQ)对脂多糖(LPS)引起的急性肺损伤(ALI)的保护作用及其分子机制。方法:HXZQ灌胃7 d后,采用腹腔注射LPS24 h建立ALI动物模型,观察HXZQ对LPS诱导的ALI的保护作用。结果:HXZQ显著降低中性粒细胞浸润,表现为中性粒细胞活性标志物髓过氧化物酶(MPO)的降低;HXZQ还能抑制巨噬细胞介导的炎症,具体表现为巨噬细胞标志物CD68和促炎因子Il-6、Il-1β的降低;同时,HXZQ通过降低淋巴细胞介导的炎症发挥免疫调节作用,具体表现为细胞因子Il-17a和Gm-csf的降低。结论:HXZQ可以通过抑制免疫细胞的浸润及其分泌的炎症细胞因子来拮抗LPS诱导的ALI。我们的研究强调了HXZQ的免疫调节作用,并提示HXZQ可能是治疗ALI的新药物。Objective: To investigate the protective effect of Huoxiangzhengqi Liquid (HXZQ) on lipopolysaccharide (LPS)-induced acute lung injury (ALI) and its molecular mechanism. Methods: After 7d of HXZQ gavage, an animal model of ALI was established by intraperitoneal injection of LPS for 24 h to observe the protective effect of HXZQ on LPS-induced ALI. Results: HXZQ significantly reduced neutrophil infiltration, as evidenced by the reduction of myeloperoxidase (MPO), a marker of neutrophil activity;HXZQ also inhibited macrophage-mediated inflammation, as evidenced by the reduction of the macrophage marker CD68 and the pro-inflammatory factors Il-6 and Il-1β;at the same time, HXZQ played an immunomodulatory role by decreasing lymphocyte-mediated inflammation. This was specifically manifested by the reduction of cytokines Il-17a and Gm-csf. Conclusion: HXZQ can antagonize LPS-induced ALI by inhibiting the infiltration of immune cells and their secretion of inflammatory cytokines. Our study highlights the immunomodulatory effects of HXZQ and suggests that HXZQ may be a new drug for the treatment of ALI.
