搜索到740篇“ CERAMIDE“的相关文章
The Combined Effect of Lumenato and Ceramide in the Protection of Collagen Damage Induced by Neutrophils in Normal Human Dermal Fibroblasts
2024年
Introduction: Collagen is the primary structural protein fibroblasts produce in the skin’s extracellular matrix. Infiltration of neutrophils into the epidermis and dermis by exposure to UV causes collagen damage and contributes to photoaging. Methods: To study the combined effect of Lumenato and ceramide in preventing collagen-1 damage induced by phagocytes, we used co-cultures of normal human dermal fibroblasts (fibroblasts) and activated human neutrophils. The present study aimed to determine the protective effect of the combination of Lumenato and ceramide on fibroblast collagen-1 damage induced by neutrophils. Results: Lumenato (in the range of 6.5 - 208 μg/ml) or ceramide (in the range of 0.1 - 50 μM) inhibited the production of superoxides and MPO by TNFα-stimulated neutrophils, as well as the production of NO by LPS-stimulated macrophages in a dose-dependent manner. The combinations of Lumenato and ceramide, in low concentrations, caused synergistic prevention of fibroblasts’ collagen-1 damage induced by TNFα-activated neutrophils, detected by fluorescence immunostaining and WB analysis. MPO activity in the supernatants of the co-cultures was also synergistically inhibited. Adding Lumenato or ceramide singly or in combinations in these low concentrations to the fibroblast cultures did not affect the expression of collagen-1. The combinations of Lumenato or ceramide in these concentrations also caused a synergistic inhibition of NO production by activated macrophages. Conclusions: The results suggest that combining low concentrations of Lumenato and ceramide results in synergistic protection against fibroblasts’ collagen-1 damage induced by neutrophils, thus indicating their possible potential for enhanced skin health.
Yulia SolomonovRachel Levy
关键词:NEUTROPHILSCERAMIDE
The Effect of a Novel Complex, Composed of Ceramide, Energizing Peptide and Camu Camu Extract, on Epidermal Barrier Function and Dermal Antiaging Properties in Ex Vivo Human Skin Small Live Cohort
2024年
The skin’s primary function is to protect the body against a spectrum of environmental stressors, including mechanical insults, microorganisms, chemicals, and allergens. Located in the outermost layers, the primary structures and components responsible for the skin’s barrier function are susceptible to environmental variables, dermatological conditions, and the aging process. The ensuing alterations to structure, composition, and organizational attributes of the epidermal barrier can impact its integrity and functionality. The aim of this study was to assess the effect of a novel complex composed of a ceramide, energizing peptide, and Camu Camu extract (SUPCERATTM complex) on specific markers of epidermal barrier integrity, as well as epidermal and dermal function. All the experiments were conducted on fresh human abdominal skin explants. Intradermal production of hyaluronic acid, epidermal claudin-1, and ceramide synthase 3 expressions, as well as epidermal lipids content were assessed using specific fluorescent stainings on ex vivo skin after the application of the complex or placebo. Additionally, dermal elastase and collagenase activities were assessed using in tubo enzymatic assays. Lastly, the effect of a cosmetic cream containing SUPCERATTM complex was assessed using subjective Global Aesthetic Improvement Scale (GAIS) in a small cohort of patients after 60 days of use. The application of the SUPCERATTM complex on ex vivo skin led to significant increase in dermal hyaluronic acid content and epidermal activity of claudin-1, ceramide synthase 3 and epidermal ceramide content. Furthermore, in tubo enzymatic assays demonstrated inhibition of both dermal elastase and collagenase activities. In addition, the patient-reported results indicated significant improvements in skin quality and appearance. .
Landau MarinaLandau-Blum ShirSamuel KassirerAudrey Le MestrArmelle PerrinJulien VerdonLaurine BergeronCorinne MorelIsabelle Imbert
关键词:ANTIAGINGCERAMIDE
胶原蛋白神经酰胺脂质体的制备及质量评价
2024年
以蛋黄卵磷脂、胆固醇和神经酰胺为成膜材料,采用薄膜分散法制备胶原蛋白神经酰胺脂质体(CO-CS)。以包封率为响应值,以药脂比、膜材比、超声时间和水合时间为影响因素对工艺进行优化。结果表明,当药脂比为1:16、超声时间为8.5 min、膜材比为4.3:1时,包封率可达90.73%。在此条件下,样品的粒径为(206.63±2.06) nm,聚合物分散性指数(polymer dispersity index, PDI)为0.187±0.010,Zeta电位为(-34.90±0.82) mV。避光保存60 d后,CO-CS的包封率为87.2%,粒径为(223.70±1.85) nm、PDI为0.174±0.013、Zeta电位为(-33.51±2.10) mV。结果表明,本研究制备的CO-CS包封率高,稳定性良好,制备工艺合理可行。
唐春慧张振博吴冬琴环飞张静姝张静姝
关键词:胶原蛋白响应面包封率
神经酰胺与急性缺血性脑卒中的研究进展
2024年
神经酰胺(Cer)是由细胞的质膜鞘磷脂降解产生的第二信使,是正常细胞功能所必需的脂质介质,由鞘氨醇和不同链的脂肪酸组成,且受体广泛存在于红细胞、内皮细胞、胶质细胞、免疫细胞、神经细胞上,参与氧化应激、炎症反应、信号通路传导、免疫调节等多种病理生理过程。多项研究证实Cer水平的增高与心血管疾病、2型糖尿病、脂质代谢有关,神经酰胺评分可以作为评估心血管事件严重程度及预后情况的工具。但是Cer在急性缺血性脑卒中(AIS)方面的影响研究较少。本文旨在阐述Cer的作用及其对AIS发生、发展和预后的影响。
李艳珍陈涛邓益东蔡奔驰关胜男
关键词:神经酰胺急性缺血性卒中
坚果油神经酰胺(NP)的合成及护肤功效研究
2024年
本文主要介绍坚果油神经酰胺(NP)的制备方法及其功效。坚果油神经酰胺(NP)是一种以天然来源的坚果油油脂为原料合成的神经酰胺。通过细胞实验考察了NP对HaCaT细胞的增殖能力、迁移能力及分泌MMP1的水平和RAW细胞分泌炎症因子(IL-6)、B16细胞分泌黑色素水平的影响;通过弹性蛋白酶活性抑制实验评价NP对皮肤弹性的影响。实验结果表明,NP具有抗光老化、抗炎、修复、美白、抗氧化等功效,未来在化妆品、生物医药、食品等领域有着广泛的应用前景。
叶柳刘晓君王蕾谷珞珈张悦李茵怡徐敏赵平杨超文
关键词:神经酰胺复合物
玫瑰果油神经酰胺(NP)的合成方法及其用途研究
2024年
以天然来源的玫瑰果油作为原料,合成复合的神经酰胺。通过HaCat细胞MMP-1表达量实验、B16细胞黑色素含量实验和DPPH自由基清除实验对玫瑰果油神经酰胺(NP)进行功效评价。实验结果表明,玫瑰果油神经酰胺(NP)具备抗衰老、抗氧化、美白的功效,未来在化妆品领域将有更广泛的应用前景。
王蕾刘晓君余浩铨赵平杨超文叶柳
关键词:神经酰胺性能评价
神经酰胺面霜辅助治疗面部敏感性皮肤患者的疗效观察
2024年
目的探究神经酰胺面霜辅助治疗面部敏感性皮肤的修复疗效与安全性,并分析其对皮肤屏障功能的影响。方法前瞻性研究,选取2022年6月至2023年4月在广东省第二人民医院皮肤科就诊的60例面部敏感性皮肤患者作为研究对象,按照随机数字表法分为对照组和观察组,各30例。对照组男3例、女27例,年龄22~39(27.97±4.80)岁,病程1周~5个月,给予盐酸西替利嗪片和舒敏治疗;观察组男3例、女27例,年龄19~42(31.33±6.42)岁,病程1周~5个月,在对照组基础上加用神经酰胺面霜治疗,均连续治疗4周。对比两组患者临床疗效、治疗前后面部皮肤角质层含水量、经表皮水分流失量(TEWL)、皮肤油脂含量、皮肤红斑量及不良反应发生情况。统计学方法采用t检验、χ^(2)检验。结果观察组治疗总有效率为96.67%(29/30),高于对照组的76.67%(23/30),差异有统计学意义(χ^(2)=5.192,P=0.022)。治疗前、第1周,两组患者面部皮肤角质层含水量及TEWL比较,差异均无统计学意义(均P>0.05)。第2周、第4周,观察组面部皮肤角质层含水量均高于对照组[(159.4±30.38)μs比(144.9±24.50)μs、(172.5±27.70)μs比(152.8±20.56)μs],TEWL均低于对照组[(21.00±3.05)g/m2/h比(22.87±2.87)g/m2/h、(18.93±2.98)g/m2/h比(21.93±2.73)g/m2/h],差异均有统计学意义(t=2.035、3.128、2.440、4.071,均P<0.05)。治疗前、第1周、第2周,两组患者面部皮肤油脂含量及皮肤红斑量比较,差异均无统计学意义(均P>0.05)。第4周,观察组面部皮肤油脂含量高于对照组[(5.60±2.39)%比(4.13±2.16)%],皮肤红斑量低于对照组[(12.43±1.76)mm2比(14.12±1.79)mm2],差异均有统计学意义(t=2.495、3.679,均P<0.05)。两组患者在治疗过程中均未出现明显不良反应。结论神经酰胺面霜对面部敏感性皮肤具有较好的辅助修复疗效,能够减轻患者临床症状,在促进皮肤屏障功能恢复方面具有一定的应用价值,且不良反应少,安全性较�
许洁如吕平平太美灵江岭李宛钊李华润秦思温炬
关键词:敏感性皮肤疗效安全性皮肤屏障功能
C2神经酰胺对帕金森病模型鼠多巴胺能神经元的保护作用
2024年
背景:C2神经酰胺可减少Alpha突触核蛋白(Alpha-Synuclein,α-Syn)寡聚体的形成,其作为蛋白磷酸酶2A激动剂在中枢神经系统对细胞老化具有重要调节作用。目的:探究C2神经酰胺对多巴胺能神经元的保护作用机制。方法:将25只C57BL/6系小鼠随机分为对照组、模型组及C2神经酰胺低、中、高剂量组,每组5只。除对照组之外,其他各组均通过左侧纹状体注射突变型A53Tα-Syn寡聚体建立帕金森病小鼠模型,在纹状体注射第30天后,3个C2神经酰胺各剂量组小鼠一次性经胃灌注溶于生理盐水中的各剂量C2神经酰胺(1,5,10μg/g),对照组及模型组同法灌注等量生理盐水,于造模后第30-90天,连续每天给药,共60 d,期间观察各组小鼠行为学变化。在纹状体注射第90天后,在适度麻醉条件下对各组小鼠灌注取脑,以免疫组织化学染色分析小鼠中脑黑质多巴胺能神经元的变化,以ELISA法检测小鼠中脑α-Syn寡聚化和磷酸化水平,并分析影响α-Syn磷酸化的相关酶活性变化。结果与结论:①C2神经酰胺对经纹状体注射的突变型A53Tα-Syn寡聚体所导致的小鼠帕金森病样运动障碍具有改善作用,且C2神经酰胺高剂量组对帕金森病模型鼠运动障碍的改善作用更为明显(P<0.01);②突变型A53Tα-Syn寡聚体致使小鼠中脑黑质多巴胺能神经元数目明显减少(P<0.01),而C2神经酰胺高剂量组黑质多巴胺能神经元数目则明显增多(P<0.01);③与模型组相比,C2神经酰胺高剂量组小鼠中脑内α-Syn寡聚体和磷酸化α-Syn水平明显降低(P<0.01),而神经酰胺的水平增高(P<0.05),蛋白磷酸酶2A活力明显上调(P<0.01);④上述数据说明,C2神经酰胺可通过改善小鼠中脑组织α-Syn的磷酸化修饰环境,缓解了由寡聚的α-Syn所诱导的神经毒性作用,保护了小鼠中脑黑质多巴胺能神经元数量的减少,从而减轻帕金森病的运动障碍程度。
李家慧齐雪朱远峰禹璐刘立峰王鹏
关键词:帕金森病蛋白磷酸酶2A
魔芋神经酰胺提取工艺及功能应用
2024年
植物神经酰胺较动物源的安全,是一种新兴的活性成分,具有屏障、保湿、抗肿瘤和提高免疫等作用,在化妆品、食品以及医药领域具有较大的发展潜力。魔芋富含神经酰胺类物质,其含量是大豆、小麦等其它植物源含量的10倍。近年,魔芋神经酰胺引起了人们极大兴趣,逐渐成为一个研究热点。本文对魔芋神经酰胺的化学结构、提取工艺以及功能应用进行综述,旨在为它的深入研究与开发利用提供参考。
邹涛王彩云张翔宇柳敏阮培均
关键词:魔芋神经酰胺化学结构
Intervention effect of Cigu Xiaozhi prescription(慈菇消脂方)on ceramide lipoapoptosis in non-alcoholic fatty liver disease
2024年
OBJECTIVE:To explore the mechanism of the Chinese medicine Cigu Xiaozhi prescription(慈菇消脂方,CGXZ)in the treatment of the non-alcoholic fatty liver disease(NAFLD)by detoxification and phlegm-reducing,the effect of CGXZ prescription on ceramide-mediated lipid apoptosis in Hep G2 cells with NAFLD.METHODS:The experiment was randomly divided into 6groups:normal control group,model group,CGXZ prescription medicated serum high,medium,and low dose groups,and pioglitazone positive control group.Using 500μmol/L free fatty acid(FFA)mixture to induce Hep G2 cells to establish NAFLD cell model,respectively,with 2%,4%,and 6%concentration of CGXZ prescription medicated serum intervention for 24 h.The changes in organelles and lipid droplet accumulation were observed under the electron microscope.Furthermore,Td T-mediated d UTP Nick-End Labeling method was used to assay hepatocyte apoptosis;Biochemical determination of glutamic-pyruvic transaminase,glutamic oxalacetic transaminase,triglycerides,and FFA levels in Hep G2cells;the content of ceramide was determined by highperformance thin-layer chromatography.Finally,Western Blot and quantitative real-time polymerase chain reaction(q RT-PCR)were used to determine the protein and gene expression levels,such as inducible nitric oxide synthase(i NOS),nuclear factorκB(NF-κB),B cell lymphoma 2(Bcl-2)and Bcl-2-associated X(Bax).RESULTS:Under the electron microscope,the cells in the model group showed moderate-to-severe steatosis,and apoptotic bodies could be seen.The model group had greater improvements in the apoptosis rate(P<0.01),and the levels of ceramide C2 and FFA in the cytoplasm(P<0.01)than the normal control group.The protein expressions of NF-κB,i NOS,and Bax were significantly up-regulated(P<0.05),while the Bcl-2 had no significant change(P>0.05).Compared with the model group,the levels of ceramide C2 and FFA(P<0.01),the protein expressions of NF-κB,i NOS,and Bax(P<0.05)in the CGXZ prescription treatment group and pioglitazone positive control group were signific
YANG ShaojunMA YanhuaBAI ZhouxiaYU YeFANG BuwuZHANG LiWANG Li
关键词:CERAMIDES

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