Three-dimensional(3D)cell cultures have contributed to a variety of biological research fields by filling the gap between monolayers and animal models.The modern optical sectioning microscopic methods make it possible to probe the complexity of 3D cell cultures but are limited by the inherent opaqueness.While tissue optical clearing methods have emerged as powerful tools for investigating whole-mount tissues in 3D,they often have limitations,such as being too harsh for fragile 3D cell cultures,requiring complex handling protocols,or inducing tissue deformation with shrinkage or expansion.To address this issue,we proposed a modified optical clearing method for 3D cell cultures,called MACS-W,which is simple,highly efficient,and morphology-preserving.In our evaluation of MACS-W,we found that it exhibits excellent clearing capability in just 10 min,with minimal deformation,and helps drug evaluation on tumor spheroids.In summary,MACS-W is a fast,minimally-deformative and fluorescence compatible clearing method that has the potential to be widely used in the studies of 3D cell cultures.
分布式控制系统,Meet All Customers’Satisfaction(MACS),其特点自在于分散控制,集中管理。2015年发布的MACS-V6K系列控制器,是由HollySys工程控制的基础控制系统,同时也是整个海洋石油平台现役的主要系统控制器,对整个海洋石油系统所有的管理和安全的设备具有控制和监视的功能,是整个海洋石油平台智能化管理系统的主要部分。所以每年对MACS-V6操作系统的年检是至关重要的,目的就是保证它可以正常运行。因此文章首先对MACS-V6的硬件特点和软件加以说明,接着介绍了硬件和软件的运行特点,最后总结了年检时所遇到的困难问题及其具体的处理方法。