Multi-strain probiotics provide the most dependable approaches to improve health,immune response,and disease resistance in farmed fishes.In the present study,we examined the effects of multi-species probiotics on the survival,growth,immune response,and disease resistance of rohu(Labeo rohita)larvae.Newly hatched larvae from the day of first feeding(average weight of 0.003 g)were reared with multi-species probiotics having a combination of Bacillus subtilis(109 colony forming units(cfu)/mL)and Lactobacillus spp.(Lactobacillus plantarum,Lactobacillus buchneri-1011 cfu/mL)in water containing doses of 0(control-C),0.5 mL/L(treatment 1-T1),and 1.0 mL/L(treatment 2-T2)in triplicates for 90 days.After the experiment,a challenge test was performed to assess the fish's resistance to pathogenic Aeromonas veronii.Significantly higher survival was recorded in larvae of treated groups(87%in T2 and 79%in T1)compared to the control(62%).Significantly higher growth performance(weight gain and specific growth rate—SGR)was shown by the probiotic-treated larval groups compared to the control.Probiotic supplementation resulted in significantly higher counts of total viable colony(TVC)and lactic acid bacteria(LAB)in the intestine.Some immunological parameters(mucosal fold fattening,goblet cell abundance,expansion of lamina propria and enterocytes)of the gut were significantly better in probiotic-treated fish.The liver of treated fish showed irregular shape nuclei turning into regular shape and reducing spaces between the hepatic cells.Probiotic-treated fish had the highest post-challenge survival rate(90%)against A.veronii infection.The erythrocytes of challenged fish treated with probiotics had significantly lower frequencies of various nuclear and cellular abnormalities.These findings suggest that multi-species probiotic supplements could improve the survival,growth,health status,and immune response of rohu in the early stages of its development.
Zannatul FerdousMd Kabir HossainMd HadiuzzamanSMRafiquzzamanKM Abdul HalimTanvir RahmanMd Ali Reza FarukZulhisyam Abdul KariMd Shahjahan
A discrete unified gas kinetic scheme(DUGKS)is developed for multi-species flow in all flow regimes based on the Andries-Aoki-Perthame(AAP)kinetic model.Although the species collision operator in the AAP model conserves fully the mass,momentum,and energy for the mixture,it does not conserve the momentum and energy for each species due to the inter-species collisions.In this work,the species collision operator is decomposed into two parts:one part is fully conservative for the species and the other represents the excess part.With this decomposition,the kinetic equation is solved using the Strang-splitting method,in which the excess part of the collision operator is treated as a source,while the kinetic equation with the species conservative part is solved by the standard DUGKS.Particularly,the time integration of the source term is realized by either explicit or implicit Euler scheme.By this means,it is easy to extend the scheme to gas mixtures composed of Maxwell or hard-sphere molecules,while the previous DUGKS[Zhang Y,Zhu L,Wang R et al,Phys Rev E 97(5):053306,2018]of binary gases was only designed for Maxwell molecules.Several tests are performed to validate the scheme,including the shock structure under different Mach numbers and molar concentrations,the Couette flow under different mass ratios,and the pressure-driven Poiseuille flow in different flow regimes.The results are compared with those from other reliable numerical methods based on different models.And the influence of molecular model on the flow characteristics is studied.The results also show that the present DUGKS with implicit source discretization is more stable and preferable for gas mixture problems involving different flow regimes.
Real-time mixing of multi-species powder challenges Laser Metal Deposition(LMD)of Functionally Graded Materials(FGMs).The current work proposes a novel method of using a static mixer to realize rapid,uniform multi-species powder mixing.Firstly,copper powder and 316L stainless steel powder are selected to complete the powder mixing observation experiment with Scanning Electron Microscope(SEM)and Energy Dispersive Spectrometer(EDS).Secondly,computational fluid dynamics and particle mixing simulation models are used to analyze the flow field and particle motion characteristics in the static mixer.Finally,LMD experiment and metallo-graphic observation are carried out with 316L stainless steel powder and WC powder to verify the feasibility of the static mixer.This study provides a theoretical and practical basis for powder mixing in laser processing with a static mixer.The conclusions can also be applied to other processing fields requiring real-time and uniform mixing of multi-species powders.