搜索到563篇“ RHODOTORULA“的相关文章
一株台湾红酵母(Rhodotorula taiwanensis)的鉴定及其除Mn(Ⅱ)性能研究被引量:1
2024年
【目的】探究一株红酵母对Mn(Ⅱ)的去除效率及其作用机制。【方法】从酸性矿山废水中分离出一株耐酸酵母菌,通过形态和26S rRNA基因测序对菌种进行鉴定,研究不同pH和Mn(Ⅱ)浓度对该菌除Mn(Ⅱ)效果的影响。采用扫描电镜、X射线衍射分析和X射线光电子能谱仪进行产物表征。【结果】分离得到的酵母菌经鉴定为台湾红酵母(Rhodotorula taiwanensis),其在pH 2.0、2000 mg/L Mn(Ⅱ)条件下仍能生长较好。在初始pH 6.0、Mn(Ⅱ)300 mg/L条件下培养144 h后,对Mn(Ⅱ)的去除率能达到98.52%;然而较高浓度的Mn(Ⅱ)(≥500 mg/L)会对细胞产生毒性,从而降低去除效果。R.taiwanensis MF4在去除Mn(Ⅱ)的过程中可以将Mn(Ⅱ)氧化成锰氧化物(主要为无定型的MnO2、Mn2O3、MnO),形成层状物质在细胞表面积累,而且能产生碱度,提升环境pH值,最高可达8.4[初始pH 7.0,Mn(II)100 mg/L,144 h]。【结论】R.taiwanensis MF4具有耐受低pH和高浓度Mn(II)、有效去除Mn(II)以及产碱的作用,研究结果对酸性矿山废水修复与治理的末端工艺设计具有参考价值。
贺笑岳正波尹家顺王绍平刘文胜张先昂揣新王进
关键词:锰离子红酵母酸性矿山废水
Marine yeast Rhodotorula paludigena VA 242 a pigment enhancing feed additive for the Ornamental Fish Koi Carp
2024年
Skin colour is an important criterion in determining the aesthetic appeal of ornamental fishes,and thus their market worth.Hence the pigment enhancement in ornamental fishes has been the subject of extensive research by using various synthetic as well as natural pigment sources.Dietary composition is one of the most important elements that influence the development of pigmentation in fish.This study is an attempt to assess the possibility of using carotenogenic marine yeast as pigment enhancer in fish diet for Koi carp,Cyprinus carpio.The 60 days feeding experiment was done by using feed incorporated with mangrove associated marine yeast Rhodotorula paludigena VA242.Greater pigmentation in the scales of fishes fed with experimental feed was observed than that fed with the control diet(normal pellet feed).Furthermore the improved growth rate and general wellbeing in fishes fed with experimental feed provides scope for further studies in this field with respect to the growth and survival of fishes.
R.RekhaK.A.NimsiK.ManjushaT.K.Sirajudheen
Engineering a non-model yeast Rhodotorula mucilaginosa for terpenoids synthesis
2024年
Terpenoids have tremendous biological activities and are widely employed in food,healthcare and pharmaceutical industries.Using synthetic biology to product terpenoids from microbial cell factories presents a promising alternative route compared to conventional methods such as chemical synthesis or phytoextraction.The red yeast Rhodotorula mucilaginosa has been widely studied due to its natural production capacity of carotenoid and lipids,indicating a strong endogenous isoprene pathway with readily available metabolic intermediates.This study constructed several engineered strains of R.mucilaginosa with the aim of producing different terpenoids.Monoterpeneα-terpineol was produced by expressing theα-terpineol synthase from Vitis vinifera.The titer ofα-terpineol was further enhanced to 0.39 mg/L by overexpressing the endogenous rate-limiting gene of the MVA pathway.Overexpression ofα-farnesene synthase from Malus domestica,in combination with MVA pathway rate-limiting gene resulted in significant increase inα-farnesene production,reaching a titer of 822 mg/L.The carotenoid degradation productβ-ionone was produced at a titer of 0.87 mg/L by expressing theβ-ionone synthase from Petunia hybrida.This study demonstrates the potential of R.mucilaginosa as a platform host for the direct biosynthesis of various terpenoids and provides insights for further development of such platforms.
Qiongqiong ChenLiting LyuHaizhao XueAabid Manzoor ShahZongbao Kent Zhao
关键词:Α-TERPINEOLΒ-IONONEΑ-FARNESENE
四类发酵助剂对红酵母产类胡萝卜素的影响
2024年
为了提高红酵母JAASSRY(Rhodotorula.glutinis JAASSRY)类胡萝卜素的合成能力,通过在发酵过程中添加4种不同类型的发酵助剂,以类胡萝卜素含量和菌体生物量为标准,探究最佳发酵助剂的种类及添加量。结果表明,适量添加发酵助剂能够有效提升红酵母的类胡萝卜素合成能力,其中维生素B族的维B_(1)(2.8 mg/L)和油脂类的大豆油(0.6 mL/L)是最适宜的发酵助剂,2种助剂的类胡萝卜素产量分别达到了224.01μg/g和325.85μg/g,生物量分别为1.30 g/L和0.90 g/L,并且在提升合成能力的同时还具有成本低、稳定性高的特点。添加维B_(1)(2.8 mg/L)和大豆油(0.6 mL/L)能够显著提升红酵母JAASSRY菌株的类胡萝卜素合成能力,是较为理想的红酵母发酵助剂。
何宇光罗正阳李达苗欣宇孙慕白牛红红华梅苏颖王景会
关键词:红酵母类胡萝卜素
海洋小红酵母H117利用高盐有机废弃物的特性分析
2024年
高盐有机废弃物属于难处理的污染物,为探究利用微生物处理高盐有机废弃物的可行性,本研究以海洋小红酵母H117为研究对象,经形态观察、生理生化、26S rRNA序列分析,确定为小红酵母(Rhodotorula minuta)。在此基础上,通过海洋小红酵母H117在高盐模拟有机废水、高盐酱油废水、高盐酱油残渣中培养后的菌落计数分析,发现其能耐受9%(m/v)盐浓度;以高盐酱油残渣为基料生长良好,最大活菌数为2.91×10^(9)cfu/mL,而在高盐酱油废水中菌落数目略少。本研究为海洋小红酵母H117转化高盐有机废弃物制备海水养殖用微生态制剂的深入研究提供了数据支撑。
温瑞玲仉倩程仕伟陈卫卫曲冠云王新杰葛宜和
关键词:耐盐工业废水微生物转化
基于菌藻混合培养合成天然类胡萝卜素的工艺研究及转录组水平分析
2024年
为突破单菌培养在微生物类胡萝卜素生产中产率低的问题,采用5 L发酵罐等体积接种高活性粘红酵母和小球藻,研究菌藻混合培养工艺的可行性,进一步从转录水平分析混合体系特定时间的菌藻协同代谢分子机制。结果表明,混合培养可获得最大生物量、残糖利用率、类胡萝卜素产量分别为51.7 g/L、83.3%和29.8 mg/L,实验组类胡萝卜素产量比菌藻纯培养组分别提高了21.5%和77.2%,且混合培养可获得7种类胡萝卜素,提高了玉米黄质和虾青素的占比;发酵36 h时取样测定分析转录组数据发现,实验组与菌藻对照组对比分别获得85个和2858个差异表达基因,GO聚类和KEGG代谢通路分析表明,小球藻可通过强化光合作用和特定类胡萝卜素合成相关基因的上调,实现兼养和改变类胡萝卜素种类的占比。
张志平段晨阳魏湘楠宋丽丽魏涛
关键词:粘红酵母小球藻转录组
深海胶红酵母的低温适应性机制初步解析
2024年
本研究以1株来源于西太平洋雅浦海沟的深海胶红酵母Rhodotorula mucilaginosa CTD02为研究对象,通过对其生理特征测定以及基因组、转录组分析,初步解析深海胶红酵母的低温适应性机制。生长曲线显示,在迟缓期适应之后,菌株可以在4℃低温环境下稳定生长进入对数生长期,体现出对低温环境的良好适应;与常温相比,低温环境下菌株细胞膜脂肪酸组成发生明显变化,不饱和脂肪酸种类和占比增加,达到了90.57%,提示菌株通过提高细胞膜的流动性来适应低温环境;不同温度下的转录组差异表达分析表明,低温上调的基因主要集中在跨膜运输、水解酶等功能,提示低温环境下菌株需要提高营养物质的运输能力来支撑细胞代谢。
许婉馨范睿凌铭煌魏士平徐志杰张恺骆祝华
关键词:深海
利用新型红酵母苯丙氨酸解氨酶制备低苯丙氨酸酪蛋白
2024年
【目的】挖掘高活性、高稳定性的苯丙氨酸解氨酶(EC 4.3.1.24;penylalanine ammonia-lyase,PAL),为后续在制备无(低)苯丙氨酸特膳食品的应用奠定基础。【方法】从胶红酵母(Rhodotorula mucilaginosa)和双倒卵形红酵母(Rhodotorula diobovata)中克隆到基因RmPAL和RdPAL,并通过生物信息学分析两个酶的序列和结构特征;在大肠杆菌中异源表达纯化RmPAL和RdPAL蛋白,测定最适反应条件和底物特异性;通过高效液相色谱和苯丙氨酸试剂盒测定了RmPAL和RdPAL转化酸解酪蛋白(casein acid hydrolysate,CAH)中苯丙氨酸(L-phenylalanine,L-Phe)的能力。【结果】RmPAL和RdPAL是真菌来源的PAL,分别由3个结构域组成:MIO结构域(MIO domain)、核心结构域(core domain)和屏蔽结构域(shielding domain),活性中心具有催化氨基酸Tyr和底物特异性特征氨基酸His;RmPAL和RdPAL在溶液中均以四聚体形式存在,两个酶的最适pH和最适温度均为8.9和50℃,且具有较宽泛的pH和温度稳定性,优于黏红酵母来源的商用PAL酶;此外,两种酶均能催化L-Phe和酪氨酸(L-tyrosine,L-Tyr)反应,且对L-Phe的催化效率较高,约为L-Tyr的5倍,脱除酸解酪蛋白中L-Phe的转化率分别为88%和93%。【结论】RmPAL和RdPAL具有较强稳定性和L-Phe水解偏好性,可从食源蛋白中有效去除L-Phe。
张阿娜韩雪韩雪辛凤姣辛凤姣
关键词:苯丙酮尿症苯丙氨酸解氨酶
Improving lipid production by Rhodotorula glutinis for renewable fuel production based on machine learning
2024年
Microbial lipid fermentation encompasses intricate complex cell growth processes and heavily relies on expert experience for optimal production.Digital modeling of the fermentation process assists researchers in making intelligent decisions,employing logical reasoning and strategic planning to optimize lipid fermentation.It this study,the effects of medium components and concen-trations on lipid fermentation were investigated,first.And then,leveraging the collated data,a variety of machine learning algorithms were used to model and optimize the lipid fermentation process.The models,based on artificial neural networks and support vector machines,achieved R?values all higher than 0.93,ensuring accurate predictions of the fermentation process.Multiple linear regression was used to evaluate the respective target parameter,which were affected by the medium components of lipid fermentation.Lastly,single and multi-objective optimiza-tion were conducted for lipid fermentation using the genetic algorithm.Experimental results demonstrated the maximum biomass of 50.3 g·L^(-1) and maximum lipid concentration of 14.1 g:L^(-1) with the error between the experimental and predicted values less than 5%.The results of the multi-objective optimization reveal the synergistic and competitive relationship between biomass,lipid concentration,and conversion rate,which lay a basis for in-depth optimization and amplification.
Lihe ZhangChangwei ZhangXi ZhaoChangliu HeXu Zhang
一种胶红酵母(Rhodotorula mucilaginosa)菌株147及其用途、产品与方法
本发明一种Rhodotorula mucilaginosa菌株147及其用途、产品与方法属于微生物技术领域。本发明提供一种胶红酵母(Rhodotorula mucilaginosa)菌株147,其保藏编号为CCTCC N...
葛谦赵华邱静苏龙王晓菁陈翔曹彩霞陈望杨斌琨开建荣张静

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