目的研究渗透树脂的牙本质小管封闭能力以及牙本质粘接效果。方法选取2021年3月至2022年10月收集的由于正畸需要拔除的健康第三磨牙120颗,随机分为比较组和分析组,每组60颗。比较组选用Single bond Universal(SBU)粘接剂,分析组选取干燥粘接联合渗透树脂,每组每次各选20颗用于封闭牙本质小管实验,微拉伸粘接强度实验,微渗漏实验,比较2组实验情况。结果2组间牙本质小管封闭率、微拉伸粘接强度差异无统计学意义(P>0.05);比较组龈壁微渗漏程度低于分析组,差异有统计学意义(P<0.05)。结论渗透树脂能够对牙本质小管进行有效封闭,并且对于牙本质粘接强度不会产生鲜明影响,但将其用作脱敏剂存在加重微渗漏情况,故应结合实际情况科学选取。
Acute kidney injury(AKI)is a significant global health issue with limited current treatment options.This study focused on the mechanism by which exosomes derived from bone marrow mesenchymal stem cells(BMSCs)promote renal tubule regeneration in AKI through the regulation of the PTEN signaling pathway by miR-21.BMSCs were isolated and characterized,and their exosomes were purified.In vitro,renal tubular epithelial cell injury models were established,and the co-culture of exosomes and cells demonstrated enhanced cell proliferation and reduced apoptosis.In vivo,AKI animal models showed improved renal function and histopathological changes after exosome treatment.miR-21 was found to be upregulated in exosomes and recipient cells,targeting PTEN and activating the PI3K/AKT pathway.The signaling network also interacted with other pathways related to renal tubule regeneration.The study highlights the potential of exosome therapy for AKI and provides insights into the underlying molecular mechanisms,although further research is needed to address remaining challenges and translate these findings into clinical applications.
Dentine hypersensitivity is an annoying worldwide disease,yet its mechanism remains unclear.The long-used hydrodynamic theory,a stimuli-induced fluid-flow process,describes the pain processes.However,no experimental evidence supports the statements.Here,we demonstrate that stimuli-induced directional cation transport,rather than fluid-flow,through dentinal tubules actually leads to dentine hypersensitivity.The in vitro/in vivo electro-chemical and electro-neurophysiological approaches reveal the cation current through the nanoconfined negatively charged dentinal tubules coming from external stimuli(pressure,pH,and temperature)on dentin surface and further triggering the nerve impulses causing the dentine hypersensitivity.Furthermore,the cationic-hydrogels blocked dentinal tubules could significantly reduce the stimuli-triggered nerve action potentials and the anionhydrogels counterpart enhances those,supporting the cation-flow transducing dentine hypersensitivity.Therefore,the inspired ion-blocking desensitizing therapies have achieved remarkable pain relief in clinical applications.The proposed mechanism would enrich the basic knowledge of dentistry and further foster breakthrough initiatives in hypersensitivity mitigation and cure.
