[Objective] This study was conducted to investigate the effects of Echi-nacea purpurea polysaccharides (EPS) on proliferation of rat intestinal epithelial cel IEC-6. [Method] The proliferation rate of IEC-6 cel s cultured in EPS at different concentrations and for different time was measured by MTT assay and analyzed by statistic methods. [Result] The proliferation rate of IEC-6 cel s cultured in EPS at al the concentrations and for different time was improved by different extents in com-parison with the control. In detail, 50 and 200 μg/ml EPS greatly improved the IEC-6 cel proliferation after 24 h of culture; then, the cel proliferation rate in the two treatments increased from 24 to 48 h, and declined from 48 to 72 h. The cel pro-liferation was also significantly improved by culturing in 100 μg/ml EPS for 72 h and in 500 μg/ml EPS for 48 h. After 48 h of culture, the proliferation rate of IEC-6 cel increased in a EPS dose-dependent manner. [Conclusion] EPS can promote IEC-6 cel proliferation, and thus improve the intestinal mucosal absorption and immune function of rat.
[Objective] This study aimed to explore the histological effects of Guchangcuzhang powder on the parenchymatous organs in experiment piglets with spleen weakness. [Method] For the 12 healthy piglets, 0.5 ml of reserpine was injected at their jugular muscle once every day for 10 days. Afterwards, the 12 piglets with diarrhea were divided into two groups, namely the Guchangcuzhang powder group and the control group, six in each. In the former group, 6 g of Guchangcuzhang powder was brewed with 20 ml of boiling water and then the piglets were drenched with the medication solution for 7 d once every day; in the latter group, each piglet was drenched with 20 ml of normal saline once every day for 7 d. On two and eight days after the drug discontinuance respectively, three piglets were selected from each of the group and killed by bloodletting from the jugular vein. Afterwards, paraffin sections of heart, liver, lungs and kidney was made and then stained respectively, followed by observation of the histological changes.[Result] The histological structure of each parenchymatous organ in piglets drenched with Guchangcuzhang powder restored or approached to normal. [Conlusion]Guchangcuzhang powder has a good therapeutic against diarrhea in piglets.