SSH was used to analyze gene transactivation during root formation of Larix cuttings. Two subtractive cDNA libraries were constructed from clone 31-6 as tester or driver and clone 15-4 as driver or tester. The SSH PCR products from the libraries were cloned into a pGEM-T easy vector and after PCR and dot blot analysis, positive clones were selected, sequenced and compared to the database in GenBank with BLASTX. The results of a sequence assembly in two libraries show that 521 UniEST （expressed sequence tag） were obtained. These 521 UniEST belong to metabolism, signal pathways, transport, resistance, developmental processes, local- ization, unknown proteins and ＂no hits found＂. All of these suggest that subtractive cDNA libraries during root formation of Larix cuttings were constructed successfully.
An E-class MADS-box gene, PdMADS2, was isolated from Populus deltoides Bartr. ex Marsh male floral buds by RT-...
BINGYU ZHANG XIANGHUA ZHANG HONG LI XIAOHUA SU (Lab of Tree Breeding and Cultivation, State Forestry Administration, Research Institute of Forestry, Chinese Academy of Forestry, Wan Shou Shan Beijing 100091) XIANGMING ZHOU(Tianjin Research Center of Agricultural Biotechnology, Baidi Road 268, Nankai District Tianjin 300192)
