Highly porous ultrafine electrospun scaffolds,gelatin/poly(L-lactic acid)(PLLA) and chitosan/PLLA were prepared by blending gelatin and PLLA,chitosan and PLLA respectively.The biocompatibilities of these scaffolds were assessed by attachment,proliferation and viability of cells on them.The results indicated that over 30%WI-38 cells could attach to the gelatin/PLLA and chitosan/PLLA scaffolds at 2 h after seeding,while the attachment of the cells was only 15%on PLLA scaffolds.Both gelatin/PLLA and chitosan/PLLA scaffolds also exhibited a very good ability for proliferation of WI-38 cells.Cell growth on the gelatin/PLLA and chitosan /PLLA scaffolds showed dramatic improvement,indicating a much better biocompatibility in the blends contributed by gelatin and chitosan.3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT) assay also demonstrated gelatin/PLLA showed better ability to enhance the growth and functions of the cells.These assays suggest that the electrospun gelatin/PLLA and chitosan/PLLA scaffolds are promising biomaterials with great biocompatibility for the development of skin tissue engineering.
