To study the modulation mechanism of the middle brain of the Gekko to the locomotion, we introduced a stereotaxic method first in literature and developed an apparatus suitable for Gekko gecko, the big-gest wall and ceiling climber in nature. We defined the bregma and nasal points as reference points, selected the bilateral infraorbital margin and top point of the maxillary tooth for locating and fixing, and set up the line passing through the bregma and paralleling to a line connecting the bilateral infraorbital margin as x axis. Then, we defined a horizontal plane in the stereotaxic instrument, passing through x axis and the certain point which is 4.8 mm exactly above the nasal point, as the XOY plane; the sagittal plane, i.e. the YOZ plane, is the plane which is perpendicular to x axis and passes through the bregma; the plane, i.e. the XOZ plane, which passes through x axis and is perpendicular to the horizontal plane is the coronal plane. We designed a set of head holder which includes three parts: bilateral infraorbital margin clamps, a gecko adaptor holding the rostral side of the upper jaw. The allocation and operation of the head holder is accurate and simple, and the device is low in cost and compatible with standard stereotaxic instrument.
