[ Objective ] The aim was to explore the relationship between the Dorsal and diapause development of Delia araiqua. [ Method ] The full-length cDNA of Dorsal in D. antiqua was cloned through RACE. The similarity among deduced amino acid sequence of Dorsal cDNA and the Dorsals of other 14 insect species were compared, and the phylogenetic analysis of these Dorsals was conducted. The expression level of Dorsal gene in winter-, summer- and non-diapausing pupae was analyzed. [ Result] The full-length of Dorsal cDNA sequence, 2 412 bp, was obtained with ORF 1974 bp, which coded 657 amino acids with predicted Mw 72.9 kDa and PI 8.5. The result of similarity comparison indicated that the DaDorsal was most related to those of Drosophlia melanogaster and Drosophlia pseudoobscura. The result of semi-quantitative RT-PCR analysis showed the expression level of Dorsal gene increased in characteristic duration of winter-, summer- and non- diapansing pupae, especially at the late diapanse, which might imply its relationship to D. antiqua diapause and development. [ Conclusion] The study lays the foundation for further study on gene function of Dorsal in insect diapause and development.
Two new species, Eumenes ferrugiantennus sp. nov. from Yunnan and E. nigriscutatus sp. nov. from Chongqing and Sichuan, China, are described. A key to the species in this genus from southwestern China is provided. Types of the new species are deposited in the Institute of Entomology and Molecular Biology, Chongqing Normal University.
[Objective] This study aims to conduct cloning and sequence analysis of ADH gene in D. Antiqua. [Method] Full-length cDNA of ADH gene in D. antiqua was cloned by using RACE technology （GenBank access number： JQ666006）. Analysis of the homology, characteristics and functional domains of ADH sequence and the phy- Iogenetic relationship to other dipteran ADH were conducted. [Result] The full length of ADH cDNA is 1 088 bp containing a 771 bp of ORF, encoding 256 amino acids, with a calculated relative molecular weight of 30.80 kDa and a theoretical isoelectric point of 8.22. The deduced amino acid sequence shares the highest homology with Glossina morsitans morsitans based on homological analysis and phylogenetic analysis. [Conclusion] This study provides basis for further research of ADH gene.
Cerogria basalis（Hope, 1831） is a large-sized species in the genus Cerogria Borchmann, 1909, and was known to be distributed in Bosnia Herzegovina, Nepal and India. This species has been lacking a detailed and formal description. In past years we collected a male and four females of this species from Yunnan, China.In this paper, we describe both the adult male and female of this species in detail, especially providing diagnostic features of the antenna, ventrite and male aedeagus. We provide photographs of the dorsal and ventral view, habitat of both male and female adults, and figures detailing the diagnostic features of both male and female antenna, male abdominal ventrites and the male aedeagus. In addition, we discuss one intraspecific variation found in this species. This provides an important basis for correct identification of this species.
