The synthesis and in vitro photodynamic anticancer activity of a new photosen- sitizer, tetra（trifluoroethoxy） germanium phthalocyanine （GePcF）, were studied. GePcF was characterized by UV-Vis, IR, MS and elemental analysis. The in vitro photodynamic activity of GePcF was studied by MTT. IC50 of GePcF for SW480 cells of human colonic adenocarcinoma and HeLa cells of cervical cancer were 36.53 and 45.78 μmol/L, respectively. GePcF as a photosensitizer may be used to treat cancers due to its photodyrmmic anticancer activity.
目的：测定和鉴别福建和广西产地金线莲中4种黄酮类化合物（异鼠李素‐3‐O‐新橙皮糖苷、异槲皮苷、槲皮素和异鼠李素）。方法应用高效液相色谱‐电喷雾电离／离子阱质谱法（HPLC‐ESI／MS）测定和鉴别4种黄酮类化合物。色谱条件：Agilent TC‐C18柱（4．6 mm ×250 mm ，5μm）；流动相：A为0．8％乙酸＋0．2％氨水溶液，B为甲醇；流速：1．0 mL／min；梯度洗脱：0～10 min ，50％～58％ B；10．01～30 min ，65％ B。检测波长368 nm ，柱温30℃，进样量20μL。质谱条件：电喷雾离子源（ESI源），正离子监测。结果不同产地金线莲中异鼠李素‐3‐O‐新橙皮糖苷、异槲皮苷、槲皮素和异鼠李素含量分别是：福建产地为 0．094，0．053，0．039和0．070 mg／g ；广西产地为0．089，0．067，0．042和0．061 mg／g。结论 HPLC‐ESI／MS法分析金线莲中4种黄酮类物质具有快速、简便的特点，且不同产地金线莲中这4种具有重要药理活性黄酮类化合物含量有差异，首次发现金线莲中含有异鼠李素‐3‐O‐新橙皮糖苷。
