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国家自然科学基金(20907017)

作品数:4 被引量:21H指数:2
相关作者:李佳姚晶周群芳梁勇林燕更多>>
相关机构:华中农业大学中国科学院生态环境研究中心江汉大学更多>>
发文基金:国家自然科学基金国家重点基础研究发展计划国家高技术研究发展计划更多>>
相关领域:生物学环境科学与工程一般工业技术化学工程更多>>

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Toxicity of the brominated flame retardant tris-(2,3-dibromopropyl) isocyanurate in zebrafish (Danio rerio)被引量:14
2011年
Tris-(2,3-dibromopropyl) isocyanurate (TBC) is a heterocyclic brominated flame retardant that was recently detected in the environment in China.TBC is semi-volatile and can accumulate in the lipid of some species,but little is known about its effect on aquatic organisms.We exposed adult zebrafish to 0,0.25,1 and 4mg/L TBC for 28 d and measured the effect on survival,growth,histopathology,hormone levels,enzyme activity,and gene expression.TBC exposure had no effect on survival or growth.We observed significant damage to the liver and gill,including hepatocellular swelling and fatty degeneration in the liver as well as proliferation and edema of epithelial cells in the gills.In addition,exposure to 4mg/L TBC induced proliferation of goblet cells in the intestine of both sexes,acellular areas in the testis,and thinly scattered vitellogenic granules in vitellogenic oocytes.TBC exposure had no effect on the levels of thyroid hormones,testosterone,estradiol,liver superoxide dismutase activity,malondialdehyde content,and brain cholinesterase activity.By contrast,hepatic vitellogenin and cytochrome P4501A gene expression was significantly down-regulated in both male and female zebrafish in response to TBC exposure.Our results suggest that exposure to TBC causes a variety of potential reproductive and endocrine toxic effects.
ZHANG XuLI JuanCHEN MinJieWU LeZHANG ChiZHANG JieZHOU QunFangLIANG Yong
关键词:溴化阻燃剂异氰脲酸酯超氧化物歧化酶活性
Mechanism of gold nanoparticle induced simultaneously increased PCR efficiency and specificity被引量:2
2013年
Gold nanoparticles(AuNPs)have been reported to induce faster heat transfer of polymerase chain reaction(PCR)solution to enhance PCR efficiency.AuNPs can also increase the specificity of PCR by functioning analogously to single-stranded DNA binding protein(SSB).However,the simple structure of AuNPs makes it difficult to determine how AuNPs affect PCR efficiency and specificity.This study aimed to elucidate the effect of AuNPs on PCR efficiency by altering the denaturation times and annealing temperatures.In addition,comparative study of the effect of AuNPs on PCR inhibition caused by two competitive primers allowed investigation of the mechanisms of AuNP-enhanced PCR accuracy.Finally,heat-treated salmon sperm DNA was used to evaluate whether AuNPs could eliminate the inhibitory PCR effect caused by DNA impurities.This study demonstrated that enhanced thermal conductivity by AuNPs was the main mechanism for increased PCR efficiency and specificity.AuNPs promoted efficient double-stranded DNA template unwinding and dissociation between mismatched primers,DNA fragments and template,and enhanced PCR efficiency and specificity simultaneously.Thus,this significant finding suggests the future use of AuNP-assisted PCR in different fields,especially in rapid clinical diagnosis and screening by PCR.
LIN YanLI JiaYAO JingLIANG YongZHANG JieZHOU QunFangJIANG GuiBin
关键词:金纳米粒子单链DNADNA模板聚合酶链反应
三种纳米金颗粒对聚合酶链式反应扩增效率和特异性的影响被引量:5
2012年
比较了采用柠檬酸三钠(Trisodium citrate,Na3Ct)还原法、聚乙烯吡咯烷酮(Polyvinylpyrrolidone,PVP)包裹法和巯基丙酸-同型半胱氨酸(Mercaptopropionic Acid-Homocystine,MPA-HCys)包裹法制备的3种纳米金颗粒(Gold nanoparticles,AuNPs)对聚合酶链式反应(Polymerase Chain Reaction,PCR)扩增效率及特异性的影响,以评估金纳米材料表面性质与DNA生物大分子之间的相互作用.实验结果显示,3种AuNPs均能显著提高PCR效率,它们提高PCR效率的能力依次为:Na3 Ct-AuNPs(12 nm)>MPA-HCys-AuNPs(12 nm)>PVP-AuNPs(4 nm),而且这种增强效应与其浓度和尺寸相关.相同实验条件下,具有相似尺寸大小的Na3Ct-AuNPs比MPA-HCys-AuNPs更能有效提高PCR效率,这说明AuNPs的表面性质也是影响PCR效率的重要因素之一.另外,3种AuNPs均能有效消除竞争性引物对PCR的抑制作用,并且这种作用与其浓度密切相关.上述研究结果表明,具有不同表面性质的3种AuNPs均能有效提高PCR效率和特异性,并且这种作用与其浓度、尺寸和表面性质密切相关.
姚晶林燕李佳周群芳梁勇
关键词:PCR特异性DNA
Induced temperature-dependent DNA degradation by C_(60) without photoactivation
2011年
This study shows that C_(60) can degrade pBR322 plasmid DNA at room temperature without photoactivation. The degradation was enhanced by increasing incubation temperature, reaction time or C_(60) concentration. We also found that superoxide radical anions (O 2 · ) were formed in the C_(60) solution. Superoxide dismutase significantly inhibited DNA cleavage and O 2 · generation induced by C_(60) . These results suggest that DNA cleavage was caused by the formation of reactive oxygen species induced by C_(60) at room temperature. Furthermore, we demonstrate that the DNA degradation was significantly inhibited by acid amide chemicals such as formamide, and by increased ionic strength of the reaction solution. These results indicate that the DNA conformation stability and the surface properties of C_(60) are important factors regulating DNA degradation. We propose that C_(60) can bind DNA, decrease DNA conformation stability, and induce the formation of reactive oxygen species and DNA cleavage at room temperature. Our results provide a possible explanation for the genotoxicity of C_(60) , which should be considered in future use of this particular nano-material.
WANG ChangLIN YanWANG YingLIANG YongMENG LingZhiZHANG JieZHOU QunFangJIANG GuiBin
关键词:温度依赖DNA降解DNA构象PBR322
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