In this study, the influences of various environmental factors on the degradation of deoxynivalenol （DON） in wheat grains were investigated using a triple quadrupole LC-MS/MS system. After being treated under different conditions for 150 d, DON toxin in wheat grains exhibited the degradation rate of 78.85%-87.00% and degradation half-life of 54.57-71.44 d. According to the accelerating effects of different conditions on DON degradation, natural light 〉 strong light （10 000 lx） 〉 darkness, 90% humidity 〉50% humidity, but high temperature could not accelerate the degradation of DON toxin.
