采用吸附/共价耦合的方法将氧化还原介体蒽醌-2-磺酸(AQS)固定在无机陶粒表面(醌基陶粒),并对其在无氧条件下强化偶氮染料的化学脱色特性进行了研究。研究结果表明,AQS能够被固定在无机陶粒上,其浓度为2.3μmol g 1;在无氧条件下,醌基陶粒强化酸性金黄G化学脱色的最佳条件为:Na2S浓度为3.2 mmol L 1,pH为6.0;整个反应过程遵循假一级反应动力学,并且随着固定化AQS浓度的增加,酸性金黄G的假一阶动力学常数明显增大。当加入60μmol L 1固态醌时,酸性金黄G、活性艳红X-3B、苋菜红和酸性橙7的假一阶动力学常数分别提高2.9、5.3、9.9和6.0倍,并且重复实验表明,醌基陶粒介导的偶氮染料脱色速率能够保持为最初的98%。由此可见,此种新型的固定化介体材料醌基陶粒在偶氮染料废水处理方面具有潜在的应用价值。
Bacterial decolorization of anthraquinone dye intermediates is a slow process under aerobic conditions. To speed up the process, in the present study, effects of various nutrients on 1-amino-4-bromoanthraquinone-2-sulfonic acid（ABAS） decolorization by Sphingomonas xenophaga QYY were investigated. The results showed that peptone, yeast extract and casamino acid amendments promoted ABAS bio-decolorization. In particular,the addition of peptone and casamino acids could improve the decolorization activity of strain QYY. Further experiments showed that L-proline had a more significant accelerating effect on ABAS decolorization compared with other amino acids. L-Proline not only supported cell growth, but also significantly increased the decolorization activity of strain QYY. Membrane proteins of strain QYY exhibited ABAS decolorization activities in the presence of L-proline or reduced nicotinamide adenine dinucleotide, while this behavior was not observed in the presence of other amino acids. Moreover, the positive correlation between L-proline concentration and the decolorization activity of membrane proteins was observed, indicating that L-proline plays an important role in ABAS decolorization. The above findings provide us not only a novel insight into bacterial ABAS decolorization, but also an L-proline-supplemented bioaugmentation strategy for enhancing ABAS bio-decolorization.
