Polyionic complex DHP-PDDA was prepared by reacting anionic surfactant dihexadecyl phosphate (DHP) with polycationic poly(diallyldimethyl ammonium) (PDDA). Thin films made from DHP-PDDA with incorporated hemoglobin (Hb) on pyrolytic graphite (PG) electrodes were characterized by electrochemistry and other techniques.
Ractopamine is a phenethanolamine leanness-enhancing agent recently approved as a feed additive but is not licensed for use in many countries. An indirect competitive enzyme-linked immunosorbent assay (ELISA) for the determination of rae- topamine has been optimized and characterized. This assay is based on a monoelonal antibody generated from raetopamine-hemiglutarate-bovine thyroglobulin (BTG), which was applied to the analysis of ractopamine in animal feed samples.