＜正＞Myroporum bontioides A.Gray,a member of the genus Myoporum,is a perennial evergreen shrub or arbor distribu...
Wu Lun-Xiu,Li Xian-Zhen,He Ting-Yu,Li Chun-Yuan and Gu Wen-Xiang Department of Applied Chemistry,South China Agricultural University,Guangzhou 510642 China
以芦丁为标准品,建立了紫外分光光度法测定苦槛蓝黄酮含量的方法,通过单因素与正交实验研究了苦槛蓝叶中黄酮的提取工艺。结果表明,采用AlCl3显色法适合苦槛蓝黄酮含量的测定,其稳定性、精密度、回收率的相对标准偏差RSD(relative standard deviation)分别为0.506%、1.30%、4.20%。影响苦槛蓝黄酮得率的主次因素为:温度>乙醇体积分数>提取时间>料液比,最佳提取工艺条件为:25倍量的60%的乙醇,在温度为90℃的条件下,提取2.0 h,提取3次,苦槛蓝总黄酮平均收率为5.16%。
The title compound, （2R,3R）-3,5,7-trihydroxyflavanone 3-acetate, is a flavanonol derivative which was first isolated from Myoporum bontioides A. Gray and characterized by MS, NMR and CD spectra. In addition, the structure was determined by X-ray single-crystal diffraction analysis. It crystallizes in the triclinic lattice, space group P21 with a = 10.686（3）, b = 6.862（2）, c = 11.267（3） , β = 107.46（1）, V = 788.3（4） 3, Z = 2, C17H16O7, Mr = 332.30, Dc = 1.400 g/cm3, μ（MoKα） = 0.110 mm-1, F（000） = 348, the final R = 0.0283 and wR = 0.0826 for 3428 independent reflections （Rint = 0.017） and 1500 observed ones （I 2σ（I））. The structure consists of one flavone and one water molecule. The flavone molecules form a 1D column by the p…π stacking interactions and C–H…O hydrogen bonds. The weak intermolecular/intermolecular O–H…O hydrogen bonds observed in the crystal give further rise to a complicated network structure. Primary bioassay showed that the title compound has high inhibitory activity against Magnaporthe grisea with the EC50 values of 199.41 μg/mL.
