In this paper, differential pulse voltammetry （DPV） was applied to study the effects of aluminum Al（Ⅲ） on the lactate dehydrogenase （LDH） activity. Michaelis-Menten constant （Km^NADH） and maximum velocity （vmax） in the enzyme promoting catalytic reaction of ＂pyruvate（Pyr） ＋ NADH ＋ H^＋ ↑LDH←→lactate ＋ NAD^＋＂ under different conditions by monitoring DPV reduction current of NAD^＋ were reported.
Kai An Yao Na Wang Jing Yue Zhuang Zheng Biao Yang Hai Yan Ni Quan Xu Cheng Sun Shu Ping Bi
