In order to provide molecular biological basis for breeding of excellent seedless varieties of Siraitia grosvenorii, the genetic background of 28 female triploid and male diploid individuals of S. grosvenorii was analyzed by using RAPD molecular markers. The results showed that female triploid and male diploid individuals of S. grosvenorli had abundant genetic background, with great genetic similarity cecfficients and close genetic distance. Overall, the genetic background of female triploid and male diploid individuals of S. grosvenorii exhibited relatively low complexity. Therefore, it is necessary to take corresponding measures for germplasm innovation to enrich the genetic background of seedless parents of S. grosvenorii.