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国家自然科学基金(30070200)

作品数:7 被引量:11H指数:2
相关作者:汪存信刘欲文王志勇张恒李杰更多>>
相关机构:武汉大学中南林学院更多>>
发文基金:国家自然科学基金更多>>
相关领域:理学自动化与计算机技术农业科学更多>>

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Thermogenetic curves and thermokinetics of seed germination of Robinia pseudoacaia
2001年
Seed germination process has closely relation with material transformation and energy exchange within the seed. Study on its thermal effect is important for understanding the mechanism and the influencing factors of the seed germination. The thermogenetic curves of seed germination ofRobinia pseudoacacia was measured by a new-type conductive microcalorimeter made in Wuhan University. The relationship was analyzed between the germination thermogenetic regulation and seed germination physiology. The thermogentic curves were further analyzed by thermokinetic theory to obtain the dynamic parameters and the thermokinetic model on seed germination ofRobinia pseudoacacia. The relationship of the thermogenetic power (μ w) and the germination time(h) of the germination process of 20 grainsRobinia pseudocacia seeds at 25°C wasP=208.77/[0.1937+0.8063exp(?0.06563t)]
胡云楚周培疆王存信屈松生
关键词:THERMOKINETICS
Kinetic Studies on Na^+/K^+-ATP_(ase) by Using Thermokinetic Method被引量:1
2004年
Na+/K+-ATPase (EC 3.6.1.3) is an important membrane-bound enzyme. By using microcalorimetry, the thermokinetic method was developed to kinetic studies on Na+/K+-ATPase for the first time. Compared with other ones, the method provided accurate measurements of not only thermodynamic data but also the kinetic data. At 310.15 K and pH=7.4, the molar reaction enthalpy rHm was measured as (-40.408±1.9) kJ·mol-1. The Michaelis constant Km was determined to be (0.479±0.020)×10-3 mol·L-1 and consistent with literature figure which is about 0.5×10-3 mol·L-1. The maximum velocity Vmax obtained was (0.681±0.026) 靘ol Pi·min-1·mg protein-1. All of the data have good repeatability and self-consistency. The reliability of thermokinetic method was verified by the experimental results and further confirmed by colorimetric studies. Moreover, the effect of enzyme pre-dilution on its activities was also investigated.
李霞李杰王志勇谢修银杨洗汪存信
关键词:MICROCALORIMETRYKINETIC
一种新型环状热电堆传感器被引量:1
2003年
设计了一种可以方便地增加热电偶数目和灵活调节时间常数的新型环状热电堆传感器。该热电堆由16个结构单元串联组成,每个结构单元由两个铝合金圆环组成,含有50对镍铬-康铜热电偶,整个热电堆一共由800对镍铬-康铜热电偶串联而成,通过增加结构单元的数目可以方便地增加热电偶的数目从而提高测温精度。当采用铜丝替换部分热电偶时就可以灵活地调节时间常数,适应各种应用的要求。设计的结果表明该热电堆传感器可检测的最小温差为1.6×10^(-6)~2.1×10^(-6)K,被替换的热电偶的数目在0~50对时其时间常数可在30.3~188.8s内变化。
张恒高明李杰王志勇刘欲文汪存信
关键词:热电偶时间常数
Thermokinetic Study on the Reversible Competitive Inhibition of Bovine Liver Arginase
2004年
A new thermokinetic reduced extent method for studying of the reversible competitive inhibition of single sub-strate enzyme-catalyzed reactions was proposed in this paper. The reaction that arginase-catalyzed hydrolysis of L-arginine to L-ornithine and urea and the inhibition of this reaction by the product, L-ornithine, and exogenous L-lysine were studied at 37 ℃ in 40 mmolL-1 sodium barbiturate-HCl buffer solution (pH=9.4). Michealis con-stant Km for arginine and maximum velocity Vm of the reaction were determined to be 5.14 mmolL-1 and 1.13× 10-2 mmolL-1s-1, respectively. The product inhibition constant KP and inhibitory constant KI of L-lysine were de-termined to be 1.18 and 5.6 mmolL-1, respectively. All the results have better repeatability and self-consistency and are in agreement with literature values. This new method using more direct thermal information from the proc-ess would give more reliable kinetic information than the traditional initial rate method.
谢修银汪存信王志勇
关键词:L-ARGININEL-LYSINETHERMOKINETICS
微量热法研究Na^+,K^+-ATP_(ase)酶促反应被引量:2
2002年
首次用微量热方法研究了Na+ ,K+ ATPase催化ATP水解的酶促反应 在 310 .15K ,pH =7.4的 10 0mmol·L-1Tris HCl,10 0mmol·L-1NaCl,10mmol·L-1KCl,3mmol·L-1MgCl2 缓冲溶液环境下 ,用对比进度法得出了Na+ ,K+ ATPase酶促反应的米氏常数Km值和最大反应速率Vm值 所得常数值与文献给出的相符 并用比色法得出了一致的Km和Vm值
李霞王志勇谢修银张文婷汪存信
关键词:微量热NA^+酶促反应比色法
一种新型贴壁式热电堆温度传感器
2002年
设计了一种由800对镍铬-康铜热电偶组成的新型贴壁式热电堆温度传感器,较好的解决了在有限的窨中容纳更多热电偶的问题,给出了一种计算时间常数的方法并对一台热导式热量计时间常数进行了计算。
张恒汪存信李杰高明刘欲文
关键词:温度传感器热电堆时间常数热电偶
水合草酸锌热分解反应动力学的主曲线法研究被引量:7
2004年
采用热重法研究了水合草酸锌热分解反应过程,并通过多升温速率法和主曲线法相结合求算得到了水合草酸锌热分解反应两个过程的动力学三因子.研究发现,水合草酸锌热分解生成氧化锌分两步进行.其中,水合草酸锌脱水反应遵循相界面控制动力学模型,草酸锌热分解生成氧化锌反应可以用一个调和的随机成核与核增长模型来描述.实验结果表明,该求算热分解反应动力学的方法能够准确、方便、全面、唯一地确定热分解反应的动力学三因子.
唐万军杨洗刘欲文汪存信
关键词:热分解
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