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国家高技术研究发展计划(2001AA620405)

作品数:2 被引量:6H指数:1
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黄海繁茂膜海绵细胞培养中抗生素的使用被引量:1
2004年
以黄海海域的繁茂膜海绵(Hymeniacidonperleve)为研究对象,考察了海绵细胞培养中细胞团的形成过程。细胞在培养24h后贴壁聚集,有类似胶原物质分泌。培养第5天形成的细胞团,中心致密,边缘有平滑的表皮结构。为控制培养中的微生物污染,比较了6种抗生素的使用效果,其中庆大霉素有较好的抑菌效果且不影响细胞成团,而两性霉素B对细胞的贴壁和成团有不利影响。
方丽驹赵权宇金美芳虞星炬张卫袁权
关键词:细胞培养抗生素两性霉素B庆大霉素抑菌效果
The Antioxidant Effects of Complexes of Tilapia Fish Skin Collagen and Different Marine Oligosaccharides被引量:5
2010年
An excess of reactive oxygen species(ROS)leads to a variety of chronic health problems.As potent antioxidants,marine bioactive extracts containing oligosaccharides and peptides have been extensively studied.Recently,there is a growing interest in protein-polysaccharide complexes because of their potential uses in pharmaceutical and food industries.However,only few studies are available on the antioxidant activities of such complexes,in terms of their ROS scavenging capability.In this study,we combined and superoxide radicals,and to evaluate the influences on the activities of superoxide dismutase(SOD),glutathione peroxidase(GSH-Px)and the level of malondialdehyde(MDA)in UV-induced photoaging models.The results indicated that the antioxidant activities of all the complexes were stronger than those of their individual components.Among the 11 complexes tested,two complexes,namely MA1000+CP and κ-ca3000+CP,turned out to be highly effective antioxidants.Although the detailed mechanisms of this improved scavenging ability are not fully understood,this work provides insights into the design of highly efficient peptide-oligosaccharide complexes for potential applications in pharmaceutical,cosmetics and food industries.
REN Shuwen LI Jing GUAN Huashi
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