高分辨核磁共振(Nuclear Magnetic Resonance,NMR)波谱是一种能够在非侵入性状态下获得生物组织分子信息的有效手段。分子间多量子相干(intermolecular Multiple-Quantum Coherence,iMQC)信号源于分子间的远程偶极相互作用,仅依赖于偶极相关距离内的磁场相对均匀性,因此可用于在不均匀磁场下获得高分辨的NMR谱。文中简要介绍了iMQC的发展历程及现状,并主要讨论了iMQC方法及其在活体及生物组织中的应用。虽然iMQC目前应用在老鼠身上比较常见,但最近的研究表明iMQC方法已被用于人体小脑的研究,将来iMQC方法将有可能应用于人体的其他器官以及其他的动物研究,具有广阔的发展前景。
In many cases, high-resolution nuclear magnetic resonance (NMR) spectra are virtually impossible to obtain by con- ventional nuclear magnetic resonance methods because of inhomogeneity of magnetic field and inherent heterogeneity of sample. Although conventional intramolecular zero-quantum coherence (ZQC) can be used to obtain high-resolution spectrum in inhomogeneous field, the acquisition takes rather long time. In this paper, a spatially encoded intramolecular ZQC technique is proposed to fast acquire high-resolution NMR spectrum in inhomogeneous field. For the first time, the gradient-driven decoding technique is employed to selectively acquire intramolecular ZQC signals. Theoretical analyses and experimental observations demonstrate that high-resolution NMR spectral information can be retrieved within several scans even when the field inhomogeneity is severe enough to erase most spectral information. This work provides a new way to enhance the acquisition efficiency of high-resolution intramolecular ZQC spectroscopy in inhomogeneous fields.