联合运用衰减全反射(attenuated total reflectance,ATR)探头与FTIR光谱仪,测定了新鲜离体的甲状腺癌转移性颈部淋巴结20枚和非转移性淋巴结69枚的FTIR光谱,比较两组光谱13个谱带的峰位及相对峰强等28个指标,找出甲状腺癌转移性淋巴结光谱的特征。与非转移性淋巴结相比,转移性淋巴结光谱中与蛋白、脂质、糖类、核酸相关的谱带均发生了明显的变化,具体如下:(1)与蛋白质相关的谱带的相对峰强I3280/I1460(P=0.029),I1640/I1460(P=0.019),I1546/I1460(P=0.004)均显著升高;(2)脂类相关的相对峰强I1743/I1460显著降低(P=0.026),而I1400/I1460则明显增高(P=0.001);(3)与糖类相关的1165与1120cm-1处峰位P1165(P=0.019),P1120(P=0.000)发生明显蓝移(向高波数移动),相对峰强I1165/I1460显著降低(P=0.006);(4)与核酸相关的1085cm-1处峰位P1085发生明显地红移(向低波数移动)(P=0.029);(5)其他指认未明的谱带的相对峰强I1303/I1460(P=0.008),I1303/I1240(P=0.039)均明显升高。与非转移性淋巴结光谱相比较,甲癌转移的淋巴结光谱在多个谱带的峰位或相对峰强方面存在显著的差异。FTIR可应用于术中甲状腺癌转移性淋巴结的探测。
In this paper,we have investigated the formation of the third-phase in tertiary amine(N235)-PtCl62--HCl system and the microscopic phase structural evolution of platinum-loaded organic phases before and after the occurrence of the third-phase.The third-phase is characterized by various spectroscopic techniques,and the small angle X-ray scattering(SAXS) experiments demonstrate the appearance of nano-aggregates,i.e.,water-in-oil reversed micelles,in the third phase.The experimental results indicate that(1) formation of the third phase is related to the aggregation behaviors of nano-reversed micelles in which a super-concentrated hydrochloric acid formed with the H+ to H2O molar ratio being much higher than that of the conventional 37 wt% saturated hydrochloric acid.(2) The occurrence of the super-concentrated HCl results in a great amount of H+ and Cl-ions enriched and confined within the nano-water pools of W/O reversed micelles in third phase.Therefore,the coordination behaviors of platinum complex ions in that super-concentrated hydrochloric acid are very different from their corresponding behaviors in bulk aqueous solutions.It is possible that H+ ions participate in the formation of such complexes as HmPtCl6z+ in the super-concentrated hydrochloric acid.(3) The relative contents of various HmPtCl6z+ complexes are different corresponding to the H+ ion concentrations in confined nanowater pools.The association ability of the acidified tertiary amine N235 molecules(R3NH+) with various HmPtCl6z+ complexes plays an important role in affecting the platinum extraction behaviors.
HUANG Kun1,CHEN Jing1,ZHANG ChengFeng1,2,LI ZhiHong3,PAN QingHua1,SUN Yan1,LIU ShaoXuan1,LIU XueXin2,LI WeiHong1,YANG ZhanLan1,WENG ShiFu1,HU TianDou3,XU YiZhuang1 & WU JinGuang1 1 College of Chemistry and Molecular Engineering,Peking University,Beijing 100871,China
