Osteoblasts were cultured on porous scaffolds of poly(L-lactide-co-glycolide) (PLGA) and PLGA/β-tricalcium phosphate (β-TCP) to evaluate their cytocompatibility.The proliferation of the cells on both scaffolds was examined before and after in vitro degradation for 4,8 and 12 weeks under static (shaking water bath) and dynamic (cyclic loading) conditions.Results indicate that porous PLGA and PLGA/β-TCP scaffolds have good biocompatibility and can be used as effective templates for guiding the growth of osteoblasts.The degradation of the scaffolds affects the proliferation of osteoblasts and the cell viability decreased with the degradation time.
YANG YanFangTANG GongWenZHAO YunHuiZHANG YangLI XiuLanYUAN XiaoYan
In order to encapsulate and controlled-release bioactive proteins,three fibrous membranes,i.e.,poly（L-lactide-co-glycolide）（PLGA）,hybrid PLGA and chitosan（H-PLGA/CS）,and core/shell PLGA/CS （C-PLGA/CS）,were produced by emulsion electrospinning,co-electrospinning and coaxial electrospinning,respectively.Bovine serum albumin（BSA） was selected as a model protein.The loading efficiency of BSA in the PLGA membrane was 1.56%,lower than those of H-PLGA/CS（5.98%） and C-PLGA/CS（4.80%）.BSA release profiles from the three membranes showed initial burst releases in the first 7 d and then sustained release for 28 d.Cumulative releases at the end of the releasing period,28 d,from the above three membranes were nearly 63%,88% and 94%,respectively,indicating that the introduction of chitosan and the core/shell fiber structure could enhance BSA release rate.In addition,all these electrospun membranes could retain their fibrous morphologies after in vitro release of BSA for 28 d.
