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国家高技术研究发展计划(2006AA100104-17)

作品数:2 被引量:27H指数:2
相关作者:郭东全赵桂兰杨向东钱雪燕尹爱萍更多>>
相关机构:吉林省农业科学院中国科学院遗传与发育生物学研究所更多>>
发文基金:国家自然科学基金国家高技术研究发展计划吉林省科技发展计划基金更多>>
相关领域:农业科学生物学更多>>

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Histological Study on Soybean Somatic Embryogenesis被引量:10
2008年
Soybean somatic cell could induce the development of embryoid which was similar to embryo morphologically and structurally. Somatic embryogenesis system of soybean was used to conduct genetic transformation of soybean because of its several advantages such as higher transformational efficiency, beetter synchronism and fewer plant chimeras among transgenic plants. After infected with agrobacterium tumefaciens,the initiation, differentiation and development of young cotyledon embryogenic cell of soybean which was cultured on selective culture medium with kanamycin were investigated through histological study. The result showed that somatic embryo was differentiated in non-bud differentiation way. The embryogenic cells were differentiated from epidermis of explant or cells in 1 layer or 2 layers, with the division of embryogenic cells and degradation and disorganization of surrounding cells, the embryogenic cells would form embryoid with analogous suspensor structure. Later, globular embryoid would extrude from epidermis then developed into heart-shape embryo. The experiment was expected to provide theoretical reference for the construction of high transformational system of using plant somatic embryogenesis induced by young cotyledon of soybean.
赵桂兰杨向东郭东全胡赞民
关键词:SOYBEANHISTOLOGY
Resistance Analysis of the Binary Insect-resistant Transgenic Soybean to Heliothis viriplaca被引量:22
2008年
[Objective] The aim of the research was to analyze the resistance of binary insect-resistant transgenic soybean to Heliothis viriplaca.[Method]In this experiment, resistance analysis of the stabilized binary insect-resistant transgenic soybean to Heliothis viriplaca was conducted in lab and in field conditions.[Result] The results indicated that the leaves of insect-resistant transgenic soybeans T5-150 and T5-195 showed lighter damage than those of non-transgenic soybeans. Meanwhile, the Heliothis viriplaca larvae fed on leaves of these two transgenic soybeans were characterized by less leaf consumption, shortening survival day, slower development and less pupation.[Conclusion]It was concluded that insect-resistance of transgenic soybean to Heliothis viriplaca was increased dramatically and the research provided a reference for selecting binary insect-resistant transgenic soybean to Heliothis viriplaca.
杨向东郭东全包绍君赵桂兰康岭生钱雪燕尹爱萍邢国杰
关键词:HELIOTHISTRANSGENICSOYBEANINSECTRESISTANCE
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