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北京市自然科学基金(7212008)

作品数:4 被引量:15H指数:2
相关作者:韩德民夏寅李希平李颖龚树生更多>>
相关机构:首都医科大学北京市耳鼻咽喉科研究所中国医学科学院北京协和医学院更多>>
发文基金:北京市自然科学基金首都医学发展科研基金国家科技重大专项更多>>
相关领域:医药卫生更多>>

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Virtual Temporal Bone Anatomy
2007年
Background The Visible Human Project(VHP) initiated by the U.S. National Library of Medicine has drawn much attention and interests from around the world. The Visible Chinese Human(VCH) project has started in China. The current study aims at acquiring a feasible virtual methodology for reconstructing the temporal bone of the Chinese population, which may provide an accurate 3-D model of important temporal bone structures that can be used in teaching and patient care for medical scientists and clinicians. Methods A series of sectional images of the temporal bone were generated from section slices of a female cadaver head. On each sectional image, SOIs (structures of interest) were segmented by carefully defining their contours and filling their areas with certain gray scale values. The processed volume data were then inducted into the 3D Slicer software(developed by the Surgical Planning Lab at Brigham and Women’s Hospital and the MIT AI Lab) for resegmentation and generation of a set of tagged images of the SOIs. 3D surface models of SOIs were then reconstructed from these images. Results The temporal bone and structures in the temporal bone, including the tympanic cavity, mastoid cells, sigmoid sinus and internal carotid artery, were successfully reconstructed. The orientation of and spatial relationship among these structures were easily visualized in the reconstructed surface models. Conclusion The 3D Slicer software can be used for 3- dimensional visualization of anatomic structures in the temporal bone, which will greatly facilitate the advance of knowledge and techniques critical for studying and treating disorders involving the temporal bone.
XIA Yin, LI Xi-ping, HAN De-min, Department of Otorhinolaryngology, Beijing Tongren Hospital, Capital Medical University, Beijing, China, 100730 ZHOU Guo-hong, ZHAO Yuan-yuan Biomedical Academy of Capital Medical University
Anatomic structural study of cerebellopontine angle via endoscope被引量:7
2007年
Background Minimally invasive surgery in skull base relying on searching for possible anatomic basis for endoscopic technology is controversial. The objective of this study was to observe the spatial relationships between main blood vessels and nerves in the cerebellopontine angle area and provide anatomic basis for lateral and posterior skull base minimally invasive surgery via endoscopic retrosigmoid keyhole approach.Methods This study was conducted on thirty dried adult skulls to measure the spatial relationships among the surface bony marks of posterior cranial fossa, and to locate the most appropriate drilling area for retrosigmoid keyhole approach. In addition, we used 10 formaldehyde-fixed adult cadaver specimens for simulating endoscopic retrosigmoid approach to determine the visible scope.Results The midpoint between the mastoid tip and the asterion was the best drilling point for retrosigmoid approach. A hole centered on this point with the 2.0 cm in diameter was suitable for exposing the related structures in the cerebellopontine angle. Retrosigmoid keyhole approach can decrease the pressure on the cerebellum and expose the related structures effectively which include facial nerve, vestibulocochlear nerve, trigeminal nerve, glossopharyngeal nerve, vagus nerve, accessory nerve, hypoglossal nerve, anterior inferior cerebellar artery, posterior inferior cerebellar artery and labyrinthine artery, etc.Conclusions Exact location on endoscope retrosigmoid approach can avoid dragging cerebellum during the minimally invasive surgery. The application of retrosigmoid keyhole approach will extend the application of endoscopic technology.
XIA Yin LI Xi-ping HAN De-min ZHENG Jun LONG Hai-shan SHI Jin-feng Department of Otolaryngology,Beijing Tongren Hospital,Capital Medical University
关键词:ENDOSCOPE
噪声性聋耳蜗螺旋神经节磷酸化c-Jun活性变化被引量:1
2009年
目的:通过神经损伤性噪声引起4周昆明小鼠出现暂时性阈移(TTS)和永久性阈移(PTS),探讨听觉通路N-甲基D-天冬氨酸受体(NMDAR)活性调节耳蜗螺旋神经节(CG)磷酸化c-Jun变化。方法:采用30只昆明小鼠制作噪声性聋动物模型,进行听觉脑干诱发反应听力检测,并采用免疫组织化学对耳蜗听觉通路中NMDAR关键成分(磷酸化c-Jun)的表达进行检测。结果:噪声性聋诱导PTS后8h、48h、7d、14dCG磷酸化c-Jun的相对吸收度值明显增加,而阳性细胞数依次减少。噪声性聋诱导PTS前后立即腹膜腔注射MK-801引起相似改变。而诱导TTS后48h则降至正常水平。结论:磷酸化c-Jun在噪声性聋后表达的增加具有时间相关性;MK-801通过阻断噪声暴露后传入神经递质谷氨酸,减少突触后钙内流所致的兴奋性毒性,从而保护听觉神经。因此,NMDAR可能参与了内耳损伤。
夏寅龙海珊韩德民龚树生雷厉史金凤范尔仲李颖赵庆
关键词:噪声性聋免疫细胞化学螺旋神经节
乙状窦后进路内窥镜手术解剖学研究被引量:7
2005年
目的:为乙状窦后进路相关内窥镜手术提供解剖学依据。方法:测量30例干颅骨表面标志位置关系,准确定位乙状窦后进路骨窗;利用10具甲醛固定成年头标本模拟内窥镜下乙状窦后进路,验证其可行性。结果:(1)以星点至乳突点连线中点为中心作直径2.0 cm的骨窗适于暴露桥脑小脑角区结构;(2)内镜下乙状窦后进路可在尽量减少压迫小脑的情况下充分暴露桥脑小脑角区神经、血管等重要结构。结论:精确定位乙状窦后进路骨窗能够最大限度减少牵拉小脑,对临床开展锁孔手术有指导意义。
夏寅李希平韩德民
关键词:内镜手术入路脑桥小脑角
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