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国家自然科学基金(30972996)

作品数:3 被引量:21H指数:3
相关作者:张浩刘小彭张炎吴晓杨晓健更多>>
相关机构:中山大学附属第三医院更多>>
发文基金:国家自然科学基金更多>>
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Microsurgical transverse 2-suture intussusception vasoepididymostomy: effectiveness and rationality被引量:5
2013年
Background 2-Suture longitudinal vasoepididymostomy shows superiority to transverse technique in an animal study; to date, this has not been consistently confirmed in human body. In the present study, we evaluated the effectiveness of 2-suture transverse intussusception vasoepididymostomy and compared the rationality between transverse and longitudinal techniques. Methods From May 2007 to December 2008, we performed 2-suture transverse vasoepididymostomy in 19 consecutive patients, as described by Marmar with modification. Between March 2009 and January 2010, the internal diameter of the vas lumen and the outer diameter of the epididymal tube were measured using microruler (21 patients and 37 sides). Results Three patients lost to follow-up. At the first follow-up period (ranged from 10 to 24 months), the patency rate was 56.3% (9/16) and the natural pregnancy rate was 25% (4/16). At the second follow-up period (ranged from 46 to 63 months), the patency rate was 68.8% (11/16), the natural pregnancy rate was 37.5% (6/16), respectively, and the take- home baby rate was 31.3% (5/16). The diameter of the vas lumen and the outer diameter of the epididymal tubule were (0.512±0.046) mm and (0.572±0.051) mm (P 〈0.001), respectively. Conclusion Transverse 2-suture intussusception vasoepididymostomy is still an effective technique in treating obstructive azoospermia.
ZHANG Hao HUANG Wen-tao RUAN Xing-xing LI Liao-yuan DI Jin-ming LIUXiao-peng XIAO Heng-jun GAO Xin ZHANG Yan
关键词:VASOEPIDIDYMOSTOMY
大鼠精索静脉曲张睾丸组织的蛋白质组学研究被引量:10
2014年
目的精索静脉曲张是男性不育的首要病因,本课题利用蛋白组学技术筛选精索静脉曲张大鼠睾丸中表达变化显著蛋白,探索精索静脉曲张引起不育的可能分子机制。方法雄性Wistar大鼠16只,随机分为对照组和精索静脉曲张实验组。实验组大鼠施行左肾静脉部分结扎术来诱导产生精索静脉曲张,对照组行假手术作为阴性对照。30 d后,收集全部大鼠的左侧睾丸。采用原位末端脱氧核苷酸转移酶标记(Tunel)法检测两组大鼠生精细胞凋亡情况,进而比较两组生精细胞的凋亡指数。利用双向凝胶电泳技术(2-DE)寻找两组大鼠睾丸中表达有显著性差异的蛋白质点,然后进行基质辅助激光解析离子化飞行时间质谱鉴定(MALDI-TOF MS)。结果双向凝胶电泳结果显示21个蛋白质点在两组间存在显著性差异。在精索静脉曲张大鼠睾丸中有8个蛋白质点表达上升、13个下降,这些蛋白功能涉及蛋白质合成、加工、降解,基因表达调控,信号转导,能量代谢,凋亡,自由基代谢等。Tunel证实实验组大鼠睾丸中生精细胞凋亡指数显著高于对照组,且本实验筛选出14-3-3ε、hnRNPF以及LZTFL1三种蛋白与凋亡相关。结论蛋白组学筛选出精索静脉曲张大鼠睾丸差异表达蛋白,并进一步证实精索静脉曲张促进睾丸生精细胞凋亡,其中相关凋亡蛋白对揭示精索静脉曲张引起男性不育的分子机制提供了可能。
贺情情刘小彭范年丰杨晓健张浩吴晓张炎
关键词:蛋白质组学精索静脉曲张不育凋亡
Vasal vessels preserving microsurgical vasoepididymostomy in cases of previous varicocelectomy: a case report and literature review被引量:6
2016年
Dear Editor, Vasoepididymostomy plays an important role in treating epididymal obstructive azoospermia and this procedure is becoming more popular in the developing countries Hcwever, we believe its effectiveness and safety should also be considered. Varicocelectomy is very common in treating male infertility due to palpable varicocele and decreased semen quality, as well as reduzing testicular pain? Therefore the question remains is whether simultaneous/asynchronous microsurgery of vasoepididymostomy and vai icocele ligation safe?
Yan ZhangXiao WuXiao-Jian YangHao ZhangBin Zhang
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