This paper investigates antispiral wave breakup phenomena in coupled two-dimensional FitzHugh-Nagumo cells with self-sustained oscillation via Hopf bifurcation. When the coupling strength of the active variable decreases to a critical value, wave breakup phenomenon first occurs in the antispiral core region where waves collide with each other and spontaneously break into spatiotemporal turbulence. Measurements reveal for the first time that this breakup phenomenon is due to the mechanism of antispiral Doppler instability.