[Objective] The aim was to test the consistency between two commonly used methods PCR detection and protein detection for detecting gene flow,and investigate the factors influencing the consistency.[Method] 1 769 samples of three varieties under three treatments（wind,bee and control）were detected with both methods.[Result] There was phenomenon in field that Bt gene was transferred into F1 generations but couldn＇t express,that meant,the result of PCR detection was not consistent with that of protein detection.By comparing environmental factors,it was proved that wind and bee treatments didn＇t significantly affect the expression of gene flow.However,the Bt gene expression rate in bee treatment was higher than that in wind treatment.[Conclusion] The paper will provide reference for accurate detection of gene flow.
The occurrence periods of Semiaphis heraclei Takahashi,Frankliniella sp.,Haptonchus luteolus and Microsphara linicerae Enchson wint.in Rabenh.causing damage on Flos lonicerae were investigated in F.lonicerae planting area in XinCheng county of Guangxi Province during 2008-2010,which were coincided with the occurrence periods of related phenology of local Prunus persica Rootstock.With P.persica Rootstock as indicator plant,the occurrence periods of three species of pests and one species of disease were predicted,respectively,and the method was simple and accurate,which could be the foundation for preventing these pests and diseases in the local field.
