＜正＞Although it is well known that one given enzyme is able to catalyze specific reaction efficiently,some unex...
Chao Li,Na Wang,Xing-Wen Feng,Kun Li,Xiao-Qi Yu~* Key Laboratory of Green Chemistry & Technology,Ministry of Education,College of Chemistry, Sichuan University,Chengdu 610064,P.R.China
＜正＞Molecular recognition of ATP by proteins,a subject of great importance for understanding enzymatic mechanis...
Jihong Liu~(a,b),Shuxia Cao~(a,*),Daxiong Hanc,Zhiping Zeng~c,Jiansha Lu~a,Xincheng Liao~a,Yufen Zhaoa,~(c,d*) a Key Laboratory of Chemical Biology and Organic Chemistry,Department of Chemistry,Zhengzhou University,Zhengzhou,450052,P R.China b Research Center of Agricultural Quality Standards and Testing Techniques,Henan Academy for Agricultural Sciences,Zhengzhou,450002,P R.China c The Key Laboratory for Chemical Biology of Fujian Province,Department of Chemistry and College of Chemistry and Chemical Engineering,Xiamen University,Xiamen 361005,P R China d The Key Laboratory of Bioorganic Phosphorus Chemisty & Chemical Biology,Ministry of Education, Department of chemistry,Tsinghua University,Beijing,100084,P R.China
Diisopropyl genistein-7-yl phosphate （C21H23O8P, Mr = 434.11） has been synthesized by a facile phosphorylated reaction with genistein and diisopropyl phosphite, and its structure was determined by IR, NMR, HR MS and X-ray single-crystal diffraction. The crystal belongs to monoclinic, space group P21/n, with a = 9.0690（18）, b = 9.0412（18）, c = 26.544（5）, β = 99.44（3）°, V = 2147.0（7） 3, Z = 4, Dc = 1.344 Mg/m3, μ = 0.172 mm-1, F（000） = 912, the final R = 0.0545 and wR = 0.1352. In the crystal structure, the title compound is constructed by both intramolecular （O-H···O=C） and intermolecular （O-H···O=P） hydrogen bonding as well as π-π stacking interaction.
A series of novel molecules with a cyclen(1,4,7,10-tetraazacyclododecane) moiety appended on and bearing different aromatic fragments in the structures were synthesized and characterized.The binding activities of these compounds towards DNA were systematically studied by spectroscopic,viscometric and gel electrophoresis methods.The results suggest that the stacking interaction plays an important role in improving the DNA binding ability of the compounds.The binding modes of the compounds towards DNA are also affected by the sizes of the aromatic rings.The binding interaction between binaphthyl compound 1b and several nucleosides was studied by fluorescence titration.Stacking interaction and hydrophobic interaction play the key role in such non-selective binding process.
HUANG Yu1,LIU JunLiang1,ZHANG Ji1,LIU Qiang1,HOU JiTing1,ZHANG Yu1,ZHANG DaWei2,LU QiaoSen1,CHEN ShanYong1,LIN HongHui2 & YU XiaoQi1 1 Key Laboratory of Green Chemistry and Technology,Ministry of Education
