Objective： Helicobacterpylori maintains long-term persistence in the host and combats oxidative stress via many antioxidant proteins, which are expected to be relevant to bacterial-associated gastric diseases. We aimed to investigate the expression of three essential antioxidants in H. pylori strains isolated from patients with different clinical outcomes. Methods： Forty H. py/ori strains were isolated from endoscopic biopsy specimens of gastric mucosa from 13 patients with gastric cancer, 13 with peptic ulcer, and 14 with gastritis. The expression of thioredoxin 1 （Trx1）, arginase （RocF）, and alkyl hydroperoxide reductase （AhpC） in H. py/ori was measured by real-time PCR. Comparisons among multiple sample sets were analyzed using a one-way ANOVA test. Pearson＇s correlation test was used to assess relationships among multiple continuous variables. Results： Trx1 expression of H. pylon in gastric cancer and peptic ulcer tissues was higher than that in tissues with gastritis. RocF expression of H. py/ori in gastric cancer tissues was higher than that in tissues exhibiting peptic ulcer and gastritis. However, we did not find any differences in AhpC expression in samples from patients with different clinical outcomes. The expression of Trx1 and RocF had a positive, linear correlation. The expression of Trx1 and AhpC had a positive correlation without a linear trend. We found no correlation between the expression of RocF and AhpC. Conclusions： Our observations indicate that the expression of Trx1 and RocF in H. py/ori might be related to gastric carcinogenesis. In hi. pylori, the expression of members of the antioxidant system may be correlated and relevant to gastric cancer.
Heficobacterpylori infection is related to the development of gastric diseases. Our previous studies showed that high thioredoxin-1 （Trxl） expression in H. pylori can promote gastric carcinogenesis. To explore the underlying molecular mechanisms, we performed an isobaric tags for relative and absolute quantitation （iTRAQ）-based quantitative proteomic analysis of stomach tissues from Mongolian gerbil infected with H. pylori expressing high and low Trxl Differences in the profiles of the expressed proteins were analyzed by bioinformatics and verified using Western blot analysis. We found three candidate proteins, 14-3-3α/β, glutathione-S-transferase （GST）, and heat shock protein 70 （HSP70）, in high Trxl tissues compared with low Trxl tissues and concluded that cellular stress and redox activity- related proteins were involved in the pathogenesis of gastric cancer associated with H. pylori Trxl.
