Ten mulberry species were taken as materials to detect contents of DNJ,polysaccharide and flavonoid.The results indicated that the three active components differed significantly upon species.Hongguo 2 and Shushen 1,which are of high contents of active components,were selected as materials to measure contents change of active components in fresh fruits during ripening process after full-bloom stage.The research suggested that DNJ mass fractions of Hongguo 2 and Shushen 1 on the 3rd d in full-bloom stage were 0.074 1% and 0.072 5%;DNJ declined gradually with growth of the fruit and DNJ contents in the two cultivars decreased by 49.80% and 58.90% on the 31st d in full-bloom stage,compared with the 3rd d.As mulberry grew,polysaccharide contents in Hongguo 2 and Shushen 1 were increasing and achieved peaks on the 27th d in full-bloom stage at 9.570% and 10.276%,respectively.For total flavonoids,the contents in Hongguo 2 and Shushen 1 were both of two peaks on 11th d and 27th d.The mass fractions were 0.313%,0.444%,and 0.264%,0.326%,accordingly.In addition,DNJ contents in ripe seeds of Hongguo 2 and Shushen 1 were significantly higher than those in fruit,but total flavonoid contents were lower.Polysaccharide was not detected.It can be concluded that mulberry fruits growing for 27 d can be either as excellent food or for medicine.
[Objective] This research aimed to explore the enrichment of edible fungi to the DNJ in mulberry branches.[Method] Reversed phase high performance liquid chromatography（RP-HPLC） method was used to study the enrichment of 9 varieties of edible fungi to the I-deoxynojirimycin（DNJ） in mulberry branches.[Result] The test edible fungi all could not synthesize DNJ,but Auricularia auricular,Ganoderma lucidum and Hericium erinaceus had enrichment capability to DNJ,and the enrichment was higher in pileus than in stipe.With the increasing proportion of mulberry sawdust in cultivation materials,the contents of DNJ in Ganoderma lucidum and Hericium erinaceus had increased;when the proportion of mulberry sawdust had achieved 50%,the contents of DNJ in the pileus of Hericium erinaceus and Ganoderma lucidum reached the maximum,which were 0.070 4% and 0.047 2%,respectively;afterwards,the contents of DNJ in the pileus were reduced with the increasing proportion of mulberry sawdust;when the proportion of mulberry sawdust had achieved 80%,the contents of DNJ in the pileus of Hericium erinaceus and Ganoderma lucidum had decreased to 0.047 4% and 0.032 7%,respectively.[Conclusion] This study provided theoretical basis and reference for the development of edible fungi in mulberry branches with hypoglycemic effects.
