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国家自然科学基金(30800677)

作品数:3 被引量:56H指数:3
相关作者:刘峰张红生马伯军唐海娟顾志敏更多>>
相关机构:浙江师范大学南京农业大学更多>>
发文基金:国家自然科学基金浙江省自然科学基金国家教育部博士点基金更多>>
相关领域:农业科学生物学更多>>

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水稻类钙调磷酸酶B亚基蛋白基因OsCBL8功能的初步研究被引量:7
2009年
以转正义或反义OsCBL8基因的T3代株系及非转基因对照为材料,研究了转OsCBL8基因对水稻农艺性状、内源OsCBL8基因表达和幼苗耐盐、耐旱及耐冷性的影响。结果表明,供试的16个转基因株系的株高、单株分蘖数、单株有效穗数、穗长和百粒重等性状与对照无显著差异,每穗实粒数和结实率则大都显著或极显著低于对照。部分转基因株系的半定量RT-PCR结果表明,转正义基因显著增强了植株中OsCBL8基因的表达,而转反义基因则对内源OsCBL8基因的表达具有一定的抑制作用。在盐、干旱和低温等非生物胁迫条件下的生长实验发现,1个转正义基因株系的耐盐性得到显著增强,1个转反义基因株系的耐旱性得到显著增强。讨论了OsCBL8基因在水稻耐非生物逆境方面可能的机理。
马伯军顾志敏唐海娟陈析丰刘峰张红生
关键词:水稻转基因植株耐逆性分子机理
Identification and characterization of putative CIPK genes in maize被引量:45
2011年
Calcium (Ca) plays a crucial role as a second messenger in intracellular signaling elicited by developmental and environmental cues. Calcineurin B-like proteins (CBLs) and their target proteins, CBL-interacting protein kinases (CIPKs) have emerged as a key Ca^2+-mediated signaling network in response to stresses in plants. Bioinformatic analysis was used to identify 43 putative ZmC1PK (Zea mays CIPK) genes in the genome of maize inbred line B73. Based on gene structures, these ZmCIPKs were divided into intron-rich and intron-poor groups. Phylogenetic analysis indicated that the ZmCIPK family had a high evolutionary relationship with the rice CIPK family of 30 members. Microarray data and RT-PCR assay showed that ZrnCIPK genes transcriptionally responded to abiotic stresses, and that 24, 31, 20 and 19 ZmCIPK genes were up-regulated by salt, drought, heat and cold stresses, respectively. There were different expression patterns of ZmCIPKs between cold-tolerant inbred line B73 and cold-sensitive inbred line Mo17 under cold stress. Our findings will aid further molecular dissection of biological functions of the CIPKs in maize, and provide new insight into the CBL--CIPK signaling network in plants.
Xifeng ChenZhimin GuDedong XinLiang HaoChengjie LiuJi HuangBojun MaHongsheng Zhang
关键词:MAIZEEXPRESSION
Preliminary Study on Function of Calcineurin B-Like Protein Gene OsCBL8 in Rice被引量:4
2010年
The homozygous T3 transgenic lines with sense OsCBL8 gene and antisense OsCBL8 gene obtained by agro-transformation were used to investigate the function of OsCBL8 in rice. Semi-quantitative RT-PCR showed that the expression of OsCBL8 extremely increased in sense transgenic lines, and decreased to some extents in antisense transgenic lines. Such up- and down-regulation of the OsCBL8 gene in these transgenic lines had little effects on main agronomic traits, but significantly decreased the number of filled grains per panicle and seed setting rate in some of transgenic lines. By evaluation of the tolerance to 150 mmol/L NaCl, 20% PEG6000 and low temperature treatments, and relevant physiological indices, 8F12, a sense transgenic line with high salt tolerance, and 8R14, an antisense transgenic line with high drought tolerance, were obtained, which suggests that the OsCBL8 gene is involved in the response of rice to abiotic stresses.
MA Bo-junGu Zhi-minTANG Hai-juanCHEN Xi-fengLIU FengZHANG Hong-sheng
关键词:RICETOLERANCE
水稻抗病相关基因spl5的克隆和机理研究
植物类病变(lesion mimics)突变体是一类在没有病原物入侵时就能自发产生坏死斑的突变体,这类突变往往使植株增强抗病性。目前,水稻中报道了近200个类病变突变体,54个已被鉴定和命名,其中8个突变基因spl7、s...
陈析丰金杨郝亮顾志敏潘建伟马伯军
关键词:水稻基因鉴定抗病性
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