您的位置: 专家智库 > >

国家自然科学基金(30470054)

作品数:47 被引量:507H指数:12
相关作者:任南琪李永峰郑国香邢德峰王爱杰更多>>
相关机构:哈尔滨工业大学哈尔滨师范大学东北林业大学更多>>
发文基金:国家自然科学基金国家重点基础研究发展计划国家杰出青年科学基金更多>>
相关领域:环境科学与工程化学工程生物学农业科学更多>>

文献类型

  • 46篇期刊文章
  • 1篇会议论文

领域

  • 22篇环境科学与工...
  • 7篇化学工程
  • 6篇生物学
  • 5篇农业科学
  • 4篇轻工技术与工...
  • 2篇动力工程及工...
  • 1篇建筑科学
  • 1篇自然科学总论

主题

  • 23篇制氢
  • 21篇生物制氢
  • 21篇产氢
  • 8篇发酵
  • 7篇氢细菌
  • 7篇产氢细菌
  • 6篇秸秆
  • 6篇DGGE
  • 5篇诱变
  • 5篇玉米秸
  • 5篇玉米秸秆
  • 5篇突变体
  • 5篇污泥
  • 4篇产氢菌
  • 4篇产氢特性
  • 3篇氢能
  • 3篇活性污泥
  • 3篇发酵类型
  • 3篇反应器
  • 3篇废水

机构

  • 39篇哈尔滨工业大...
  • 4篇哈尔滨师范大...
  • 4篇东北林业大学
  • 3篇哈尔滨工程大...
  • 3篇黑龙江大学
  • 2篇东北农业大学
  • 2篇上海工程技术...
  • 1篇黑龙江中医药...
  • 1篇辽宁大学
  • 1篇中国科学院
  • 1篇加拿大环境部

作者

  • 35篇任南琪
  • 11篇李永峰
  • 9篇郑国香
  • 8篇邢德峰
  • 6篇林海龙
  • 6篇王爱杰
  • 5篇宋佳秀
  • 5篇包红旭
  • 4篇李建政
  • 3篇胡立杰
  • 3篇徐香玲
  • 3篇郭婉茜
  • 3篇宋业颖
  • 3篇赵丽华
  • 2篇张汝嘉
  • 2篇徐菁利
  • 2篇许继飞
  • 2篇张文启
  • 2篇杨传平
  • 2篇王相晶

传媒

  • 8篇太阳能学报
  • 5篇哈尔滨工业大...
  • 4篇环境科学
  • 3篇中国甜菜糖业
  • 3篇化工学报
  • 2篇中国环境科学
  • 2篇Journa...
  • 1篇生态学报
  • 1篇环境科学学报
  • 1篇净水技术
  • 1篇生物技术
  • 1篇微生物学报
  • 1篇给水排水
  • 1篇微生物学通报
  • 1篇化学工程
  • 1篇中国给水排水
  • 1篇环境科学研究
  • 1篇生物工程学报
  • 1篇大连海事大学...
  • 1篇南京理工大学...

年份

  • 1篇2010
  • 7篇2009
  • 14篇2008
  • 8篇2007
  • 13篇2006
  • 4篇2005
47 条 记 录,以下是 1-10
排序方式:
DG-DGGE分析产氢发酵系统微生物群落动态及种群多样性被引量:40
2005年
应用双梯度-变性梯度凝胶电泳(DG-DGGE)对生物制氢反应器微生物种群的动态变化及多样性进行监测。间隔7d从反应器取厌氧活性污泥,以细菌16SrDNA通用引物进行V2~V3区域PCR扩增,长约450bp的PCR产物经DGGE分离后,获得污泥微生物群落的16SrDNA指纹图谱。污泥接种到反应器后微生物群落中既有原始种群的消亡和增长,也有次级种群的强化和演变。反应器在运行初期群落演替迅速,15d时微生物群落结构变化最大。群落结构的相似性随着演替时间的增加而逐渐升高,种群动态变化后形成稳定的群落结构。29d时微生物多样性基本保持不变,微生物优势种属达到19个OTU。在细菌竞争和协同作用制约下,种群多样性降低后趋于稳定,形成顶级群落。有些种群在群落结构中一直存在,是群落建成的原始种群,原始种群与次级种群在代谢过程中具有协同作用,表现出群落的综合生态特征。
邢德峰任南琪宋业颖李秋波赵立华徐香玲
关键词:群落动态群落多样性生物制氢
新型生物制氢反应器的运行及产氢特性被引量:10
2009年
以厌氧活性污泥为产氢菌种,糖蜜废水为底物,研究了新型外循环颗粒污泥膨胀床(ECGSB)生物制氢反应器的运行及产氢特性。结果表明,ECGSB反应器可在较高的容积负荷(VLR)下实现高效稳定的产氢,稳定运行时,反应器内可观察到自絮凝产氢颗粒污泥的形成,污泥平均浓度高达24.1gVSS/d,系统最大产氢能力为7.43m^3/m^3·d,发酵气中氢气体积含量为50%~56%。系统形成自絮凝产氢颗粒污泥是ECGSB反应器高效运行和产氢的关键,自絮凝产氢颗粒污泥既增加了活性产氢细菌的生物持有量,又提高了系统抗冲击负荷的能力。连续流运行各项参数表明,ECGSB反应器具有良好的运行稳定性和产氢优势;提出乙醇型发酵快速启动的调控对策,在发酵法生物制氢领域具有广泛的应用前景。
郭婉茜任南琪王相晶向文胜曲媛媛
关键词:生物制氢运行特性
超声波破碎法提取活性污泥DNA及其DGGE分析被引量:14
2008年
为快速提取活性污泥中的微生物DNA,进一步应用于变性梯度凝胶电泳(DGGE)技术分析其群落结构,采用细胞超声波破碎法直接提取反应器活性污泥DNA,以16S rRNA V3区域通用引物进行PCR扩增,随后利用DGGE技术对扩增产物的多样性进行评估.结果表明,超声波破碎法可以快速地提取活性污泥DNA,用超声波破碎法提取到的活性污泥克服了PCR扩增困难的问题.在一定的超声波功率下,超声时间对DNA产率、PCR扩增效率和DGGE分析均有很大影响,实验的最佳超声时间为27 s.DGGE分析表明,用该法提取到的DNA种类较为丰富,多样性较好,种群强度最高能达到0.7 OD,能够进一步应用于群落结构分析.
万晶晶张汝嘉邢德峰谢天卉赵丽华任南琪
关键词:活性污泥DNA提取PCR
Monitoring of microbial community structure and succession in the biohydrogen production reactor by denaturing gradient gel electrophoresis(DGGE)被引量:5
2005年
To study the structure of microbial communities in the biological hydrogen produc-tion reactor and determine the ecological function of hydrogen producing bacteria,anaerobic sludge was obtained from the continuous stirred tank reactor(CSTR)in different periods of time,and the diversity and dynamics of microbial communities were investigated by denaturing gra-dient gel electrophoresis(DGGE).The results of DGGE demonstrated that an obvious shift of microbial population happened from the beginning of star-up to the 28th day,and the ethanol type fermentation was established.After 28 days the structure of microbial community became stable,and the climax community was formed.Comparative analysis of 16S rDNA sequences from reamplifying and sequencing the prominent bands indicated that the dominant population belonged to low G+C Gram-positive bacteria(Clostridium sp.and Ethanologenbacterium sp.),β-proteobacteria(Acidovorax sp.),γ-proteobacteria(Kluyvera sp.),Bacteroides(uncultured bacte-rium SJA-168),and Spirochaetes(uncultured eubacterium E1-K13),respectively.The hydrogen production rate increased obviously with the increase of Ethanologenbacterium sp.,Clostridium sp.and uncultured Spirochaetes after 21 days,meanwhile the succession of ethanol type fer-mentation was formed.Throughout the succession the microbial diversity increased however it decreased after 21 days.Some types of Clostridium sp.Acidovorax sp.,Kluyvera sp.,and Bac-teroides were dominant populations during all periods of time.These special populations were essential for the construction of climax community.Hydrogen production efficiency was de-pendent on both hydrogen producing bacteria and other populations.It implied that the co-metabolism of microbial community played a great role of biohydrogen production in the reactors.
XING DefengREN NanqiGONG ManliLI JianzhengLI Qiubo
碳源、氮源及碳/氮比值对发酵产氢细菌RF-9产氢性能的影响
利用间歇实验对不同碳源、氮源及碳氮质量比值对产氢细菌RF-9的发酵产氢行为影响进行了探讨。结果表明,葡萄糖和玉米浆+酵母膏复合物是产氢菌RF-9发酵产氢较理想的碳源和氮源。碳/氮(mC/mN)质量比值对菌株RF-9的生长...
郑国香任南琪钟溢键李小玲吴川福周湘良
关键词:碳源氮源
文献传递
Length polymorphisms for intergenic spacer regions of 16S-23S rDNA in members of the new hydrogen-producing bacteria
2007年
A method based on PCR amplification of the 16S rRNA gene (rDNA)-23S rDNA intergenic spacer regions (ISR) was developed for the identification of species within the novel group hydrogen-producing anaerobes. The sizes of the PCR products varied from 1264 to 398 bp. Strain of isolate Rennanqilyf 3 was characterized as having products of 1262,398,638,437 and 436 bp. The isolate Rennanqilyf 1 had product of 1264 bp. The isolate Rennanqilyf 13 had products of 1261,579 and 485 bp. Of the 3 species of the novel group hydrogen-producing anaerobes examined, no one was indistinguishable. Two environmental isolates were identified as hydrogen-producing bacteria, which were new species in present taxon. Rennanqilyf 3 could not be associated with any Clostridium sp. studied. Rennanqilyf 1 could be classified into Clostridium genus. The combination between 16S rDNA equencing and length polymorphisms of IRS in 16S-23S rDNA is a better method for determining species of the hydrogen-producing bacteria.
李永峰徐菁利杨传平任南琪
Hydrogen producting characteristics by a novel strain of bacteria-ethanoligenens sp. B49被引量:2
2008年
The objective of this work is to investigate the fermentation capacity and metabolic characteristics of a novel strain of bacteria B49 isolated from anaerobic activated sludge. The examination was conducted in batch culture at 35 ℃. The results showed that the carbon flow gave priority to the production of ethanol, and yield of ethanol is always greater than that of acetic acid. The hydrogen and ethanol occurred simultaneously. The exponential phase of the B49's cell growth was from 12 to 22 h. Evolution of hydrogen appeared to start after the exponential phase of cell growth and reach maximum production at the early stationary phase. The rate of hydrogen production reached a maximum of 16.8 mL/h, and the percentage of hydrogen gas in the headspace of serum bottle obtained a maximum of 41 % at 22 h. The B49 was able to grow using molasses as substrate for cell growth. When the molasses was used as substrate, maximum yield of hydrogen was obtained 2460 mL/L culture at 2 % (V/V) of molasses. The hydrogen yield was increased to 3060 mL/L culture after addition of 0.5 g/L of yeast extract in the molasses medium and the yield of hydrogen was increased by 24.4%.
许丽英任南琪王兴祖张颖徐慧陈冠雄贾永锋
关键词:ETHANOLDYNAMICS
复合菌群降解玉米秸秆协同产氢特性
2009年
为加快生物制氢工业化进程,利用玉米秸秆这类来源广泛、储量巨大和价格低廉的可再生生物质纤维素资源作为发酵产氢的原料,从连续流发酵产氢反应器(ZL92114474.1)中新分离筛选出一株高效纤维素降解产氢细菌Clostridium sp.X9(NCBI注册号:EU434651)和一株高效产乙醇发酵产氢细菌Ethanoigenens harbinenseB2(NCBI注册号:EU639425),通过构建高效降解纤维素发酵产氢复合菌群进行同步降解玉米秸秆发酵产氢.结果表明,对玉米秸秆进行酸化汽爆预处理后可以显著提高复合菌群的产氢能力.复合菌群X9和B2比单一菌种具有更理想的降解玉米秸秆发酵产氢的能力,两菌种间存在协同产氢效应.复合菌群X9和B2降解玉米秸秆发酵产氢获得的最大产氢率和玉米秸秆降解率分别为8.7mmol/g和74%.液相代谢末端产物主要为乙醇、乙酸和丁酸.这说明复合菌群X9和B2在以木质纤维素为发酵底物的工业化生物制氢领域中具有很好的应用发展前景.
包红旭任南琪王爱杰
关键词:生物制氢复合菌群降解玉米秸秆
优势产氢-产酸发酵类型的建立及比较被引量:1
2006年
利用两套连续流搅拌槽式生物制氢反应器(CSTR),采用优化的启动参数,实现了优势产氢-产酸发酵类型(乙醇型和丁酸型)的启动和稳定运行;计算两者的投碱量,发现维持丁酸型发酵所消耗的碱是维持乙醇型发酵的7倍;在稳定运行阶段,在相同进水COD负荷情况下,两个反应器的产气及产氢能力基本相当,日产氢量在11.3-14.5 L范围内,而丁酸型发酵的生物持有量为乙醇型的1.8倍,而乙醇型发酵菌群的比产氢速率要高于丁酸型,分别为平均21.2 mol/(kgVSS·d)和11.1 mol/(kgVSS·d)。试验证明,乙醇型发酵茵群具有更宽的pH耐受幅,因此对环境的适应能力更强。
宋佳秀任南琪段志杰
关键词:生物制氢氢经济荧光原位杂交
多氯联苯在自然水体中的分布现状与处理工艺被引量:24
2006年
分析了我国自然水体中多氯联苯(PCBs)的分布现状,探讨了PCBs的生态毒性效应,并提出了控制PCBs危害的有效措施。研究结果表明,我国自然水体中PCBs超标率已达57.6%,有些水体超标倍数极高;水体的底泥是PCBs的富集场所,正成为新的PCBs污染源;我国在PCBs研究方面存在区域性不平衡问题,并且普遍缺乏系统、连续的监测数据。为有效控制PCBs的危害,应该广泛开展国际合作,完善管理体系,并建立统一的风险评估、经济评估和预警机制。
阙明学温青刘广民李一凡
关键词:水体污染
共5页<12345>
聚类工具0