[Objective] The research aimed to establish the fast and efficient rapid propagation system for Ampelopsis grossedentata by using the tissue culture method. [Method] Ampelopsis grossedentata stem with the bud was the material,and the influences of different sterilization methods,media and hormone combinations on the in vitro rapid propagation of Ampelopsis grossedentata were studied. [Result] The explant sterilization effect of 75% ethanol dipping 20 s ＋ 0.1% HgCl2 treating 8 min ＋ Tween-80 1-2 drops was better. The optimal medium for the axillary bud induction was B5 ＋ 1.00 mg/L BA ＋ 0.05 mg/L NAA. The optimal multiplication medium was MS/B5 ＋ 1.50 mg/L BA ＋ 0.05 mg/L NAA. 1/2MS ＋ 0.50 mg/L IBA was the best medium for the rooting. [Conclusion] The high frequency occurrence system of Ampelopsis grossedentata in vitro rapid propagation was established. It laid the technology basis for the callus regeneration system,genetic transformation and clonal mutation screening.
