The effect of sucrose on nanometer Se0 in liquid phase was reported.Transmission electron microscope（TEM）,laser light scattering image and Resonance Rayleigh Scattering（RRS） were used to study the configuration and stability of nanometer Se0 particles.The results show that sucrose solution modulated the assemble of nanometer Se0 particles and stabilized the Se0 particles in liquid phase.From the concentration of 1.07 to 42.8 μmol/L,the diameter of Se0 particles were 47—600 nm.However,in the presence of 1.0% sucrose solution,the major distribution range of Se0 diameter was 20—80 nm.Furthermore,the nanometer Se0 sol-gel could be preserved stably in sucrose solution at least for 2 months at 2—10 ℃.
The electrochemical character of bilirubin（BR）-M2+(M2+=Cd2+,Cu2+,Zn2+,Ca2+,Fe2+)Langmuir-Blodgett（LB）film modified glassy carbon electrode was investigated,and was compared with that of BR-LB film modified glassy carbon electrode and that of BR and M2+in solution.On BR-M2+-LB film modified glassy carbon electrodes,one anodic peak for BR-M2+ complex was observed at-679～-842mV,and the anodic peak potential of BR shifted towards to ositive from 453 mV to 477～493 mV.BR-M2+（in solution）formed BR-M2+ complex with BR on the BR-LB film modified glassy carbon electrode,at the same time,M2+permeated BR-LB film and occrred electrode reaction.The anodic peak potential of BR-M2+ complex on LB film shifted towards positive with the increasing of stability of biologic metal complex.In BR and M2+ solution,the potentials of two anodic peaks for BR（400 mV and 305 mV）was shifted positive 19～51 mV due to BR-M2+ complex forming,and this potential was effected by the ratio of BR and M2+.Moreover,a pair of symmetrical adsorption peak was appeared at nearly 0 mV,which indicated the hydrophobicity of this complex molecule was stronger than that of BR molecule.
