To develop a better approach for spatial evaluation of drinking water quality, an intelligent evaluation method integrating a geographical information system(GIS) and an ant colony clustering algorithm(ACCA) was used. Drinking water samples from 29 wells in Zhenping County, China, were collected and analyzed. 35 parameters on water quality were selected, such as chloride concentration, sulphate concentration, total hardness, nitrate concentration, fluoride concentration, turbidity, pH, chromium concentration, COD, bacterium amount, total coliforms and color. The best spatial interpolation methods for the 35 parameters were found and selected from all types of interpolation methods in GIS environment according to the minimum cross-validation errors. The ACCA was improved through three strategies, namely mixed distance function, average similitude degree and probability conversion functions. Then, the ACCA was carried out to obtain different water quality grades in the GIS environment. In the end, the result from the ACCA was compared with those from the competitive Hopfield neural network(CHNN) to validate the feasibility and effectiveness of the ACCA according to three evaluation indexes, which are stochastic sampling method, pixel amount and convergence speed. It is shown that the spatial water quality grades obtained from the ACCA were more effective, accurate and intelligent than those obtained from the CHNN.