您的位置: 专家智库 > 资助详情>国家自然科学基金(10974146)

国家自然科学基金(10974146)

作品数:5 被引量:4H指数:1
相关作者:杨光参王艳伟冉诗勇马秋梅焦卓锋更多>>
相关机构:温州大学更多>>
发文基金:国家自然科学基金浙江省自然科学基金国家重点基础研究发展计划更多>>
相关领域:理学生物学机械工程更多>>

文献类型

  • 5篇中文期刊文章

领域

  • 3篇理学
  • 2篇生物学
  • 1篇机械工程

主题

  • 3篇DNA
  • 1篇单分子操纵
  • 1篇蛋白
  • 1篇动态光散射
  • 1篇增强拉曼光谱
  • 1篇染料
  • 1篇染料分子
  • 1篇紫外
  • 1篇紫外吸收
  • 1篇紫外吸收光谱
  • 1篇组蛋白
  • 1篇吸收光谱
  • 1篇纳米
  • 1篇拉曼
  • 1篇拉曼光谱
  • 1篇拉曼散射
  • 1篇光谱
  • 1篇光散射
  • 1篇分子
  • 1篇复合物

机构

  • 3篇温州大学

作者

  • 3篇王艳伟
  • 3篇杨光参
  • 2篇焦卓锋
  • 2篇马秋梅
  • 2篇冉诗勇
  • 1篇林瑜

传媒

  • 1篇物理学报
  • 1篇浙江大学学报...
  • 1篇山东科学
  • 1篇Chines...
  • 1篇Chines...

年份

  • 1篇2017
  • 1篇2013
  • 2篇2011
  • 1篇2010
5 条 记 录,以下是 1-5
排序方式:
Analysis of the time-domain spectrum of hydrogen in electric field near helium surface被引量:1
2011年
The Ryderberg electronic wave packet dynamics of hydrogen atom near helium surface in an electric field is investigated using the semiclassical method. The autocorrelation function is calculated when the photoionized electron is excited by a short laser pulse for different atom-surface separations. The results show that new recurrences appear because of the helium surface, and the number of recurrent peaks increases with the decrease in atom-surface distance. The new feature is ascribed to the bifurcation of new closed orbits in the classical dynamics of the photoionized electron. Therefore, surface properties have a significant effect on the spectrum of nearby atoms or ions.
龚晓青杨光参
关键词:ATOMSHELIUMHYDROGENPHOTOIONIZATION
DNA平衡离子凝聚的动态光散射分析被引量:1
2013年
利用动态光散射技术系统地研究了不同化合价的平衡离子氯化钠(Na+)、氯化镁(Mg2+)、三氯六氨络合钴([Co(NH3)6]3+)和精胺([C10N4H30]4+)与DNA之间的相互作用.实验结果显示,当缓冲液中只包含Na+或Mg2+,且浓度c5mM时,DNA电泳迁移率之比约为2:1;当缓冲液单独包含Na+或[Co(NH3)6]3+,且浓度c5mM时,DNA的电泳迁移率之比约为4.5:1.而当平衡离子的化合价为4时,观察到DNA的电泳迁移率由负变正,意味着发生了电荷反转.在平衡离子浓度c<5mM时,随着离子浓度的增大,迁移率之比逐渐增大.对于一价或二价平衡离子情形,实验结果与Manning的平衡离子凝聚理论的预言相符.对于三价平衡离子,实验结果与理论值有明显偏离.而对于四价离子,由于发生了DNA电荷逆转,基于平均场的平衡离子凝聚理论失效.另外,通过原子力显微镜观察到当平衡离子的化合价大于等于3时,DNA分子的构型发生变化.因此,自由溶液中的聚电解质的构型和离子关联效应在聚电解质迁移过程中起重要作用.
林瑜杨光参王艳伟
关键词:动态光散射DNA
纳米粒子体系中染料分子的表面增强拉曼光谱被引量:1
2010年
用化学还原的方法制得银胶体,并加入酒精或腺嘌呤(A)使其聚集,以聚集的银胶体作为表面增强拉曼散射的衬底。分别用原子力显微镜和紫外分光光度计来检测银胶体聚集前后的表面形貌和吸收光谱。以罗丹明B(RhB),罗丹明6G(R6G)作为探针分子测定衬底的表面增强拉曼散射效应。结果表明,在同一衬底上,RhB的拉曼灵敏度大约是R6G的2倍。RhB在腺嘌呤引起银胶体聚集形成的衬底表面的拉曼灵敏度比在酒精引起银胶体聚集形成的衬底表面的拉曼灵敏度高。由此可知腺嘌呤引起银胶体聚集的SERS衬底上的"活性点"比酒精引起银胶体聚集的衬底上的"活性点"活跃。
马秋梅王艳伟焦卓锋冉诗勇杨光参
关键词:AG纳米粒子紫外吸收光谱
Modulation and control of DNA charge inversion
2017年
DNA is one of most important biological polyelectrolytes, which is negatively charged in physiological condition. Most of Its charge is neutralized by attracting cations in solution. In some conditions, the effective charge of DNA switches its sign from negative to positive, implying charge inversion of DNA. The underlying microscopic mechanism of the counterintuitive phenomenon is still not fully understood although specific chemical affinity and electrostatic ion correlation are considered as two possible driving forces. In this review, we present some recent experimental progress in the modulation and control of DNA charge by single molecular techniques. It has been shown that DNA charge inversion can be modulated bidirectionly by decreasing or increasing the dielectric constant of solution to make the electrophoretic mobility of DNA increase from a negative value to a positive value. In this meanwhile, charge inversion and condensation of DNA in solution of trivalent and quadrivalent counterions are significantly influenced by pH value of the solution. When mixing quadrivalent counterion with mono-, di-and tri-valent counterions in solution, suppression and promotion of DNA charge inversion can be observed. In addition, hydrophobic effect can play an important role in DNA charge inversiton and compaction. We show that the organic monovalent ions of tetraphenyl chloride arsenic (Ph4As+) can induce DNA compaction and even invert its electrophoretic mobility. Thus, hydrophobic effect can be the main driving force of DNA charge inversion and compaction by the organic monovalent ion.
王艳伟杨光参
关键词:COUNTERIONSHYDROPHOBICITY
一种单分子操纵DNA-组蛋白复合物的新方法被引量:1
2011年
介绍了在云母表面一种拉直λ-DNA-组蛋白复合物的新方法.借助原子力显微成像表明,该方法不但对裸λ-DNA的拉直效果较好,而且可以拉直不同浓度的DNA-组蛋白的复合物.利用此方法可以在单分子水平上研究DNA的复制、转录过程以及DNA-蛋白质相互作用.
焦卓锋马秋梅王艳伟冉诗勇杨光参
关键词:Λ-DNA组蛋白
共1页<1>
聚类工具0