某动物园圈养的环尾狐猴发生了一种以高烧不退,精神沉郁,活动减少等症状为特征的急性疾病。笔者对发病死亡的动物进行剖检,采集肺、脑、肝等组织,接种vero细胞,72小时后细胞出现明显病变。在病原核酸背景未知的情况下,应用非序列依赖性单引物扩增法结合DNA酶处理(DNase-sequence independent single primer amplificmion,DNase-SISPA)对病原基因进行扩增。结果显示:其中以DNA为模板的扩增产物测序后与Genbank中序列进行BLAST分析,与数据库中华诊体衣原体(Waddlia chondrophila)核苷酸序列有97%的相似性,而以cDNA为模板的扩增片段测序在NCBI中没有找到序列相似性高于50%的基因片段,初步诊断导致此次环尾狐猴疫病的病原为华诊体衣原体(Waddlia chondrophila)。
Blue and Yellow Macaw ( Ara ararauna ), which is part of Psittaciformes, Psittacidae, is distributed mainly over South America. They always dwell in the primeval forest, feed on various plant, fruit, seed and bud, and also eat some insets in their breed period. It is incorporated into the Appendices Ⅱ of Convention or international trade in endangered species of wild fauna and flora. Now there are many zoos recommend the Blue and Yellow Macaw and feed it, but never have report of breed. Guangzhou Zoo had succeeded in breeding for two years in 1997 and 1998, by improving feeding environment, mixing fodder rationally and strengthening the administration of feeding.
