In order to investigate the distribution of soil available micronutrients, and provide a theoretical basis for management of fertilizing reasonably on crops, the contents of major and trace elements (P, K, Ca, Mg, Cu, Fe, Zn, and Mn) in farm- land soil collected from Yunnan Province were determined by inductively coupled plasma-mass spectrometry (ICP-MS), the contents and distribution of major and trace elements were also studied. The results showed that the contents of available P, K were balance, available Ca, Mg were deficient, and available Cu, Fe, Zn, Mn were very plentiful. Major and trace elements distribution of different landform areas were obviously different. The contents of trace elements (Fe, Zn and Mn) of central Yunnan Red Plateau were significantly higher than those of Hengduan Mountains in western Yunnan and those of karst plateau in eastern Yunnan. The contents of available P, K, and Ca gradually declined from west to east, while the content of available Cu rose gradually from west to east. The results are reference value to elements determination and agricultural production in farmland soils.