[Objective] This study aimed to investigate the effect of inoculation with various rhizobium strains on growth status, phosphorus uptake, yield and quality of soybean plants. [Method] Using Dongnong 42 and Dongnong 46 as experimental soybeans, four treatments were designed, including non-inoculated CK group and three treatment groups inoculated respectively with rhizobium strains R2, R, and Rs, to analysis the effects of rhizobium inoculation on growth status, phosphorus uptake, yield and quality of soybean plants. [Result] Inoculation with different rhizobium strains could significantly increase the biomass and phosphorus content of the roots and shoots of Dongnong 42, to be specific, rhizobium strain R2 showed the best ef- fect, followed by Rs, and those of roots were improved greater than the shoots; in- oculation with rhizobium could significantly increase the yield of Dongnong 42, and R5 showed the best effect, but the yield of Dongnong 46 was decreased; after in- oculated with rhizobium, the protein content and fat content of soybean were signifi- cantly increased, and R2 showed the best effect, but the effects on different varieties varied, to be specific, the protein content of Dongnong 42 was significantly in- creased, while the fat content of Dongnong 46 was significantly increased; different rhizobium strains inoculated to different soybean varieties showed different effects. Inoculation with suitable rhizobium can improve plant growth characteristics, promote phosphorus uptake of plants, increase soybean yield, and effectively improve soy- bean quality. [Conclusion] This study provides theoretical basis for solving the prob- lem of soil phosphorus deficiency, increasing soybean yield and improving soybean quality in the future.