Effects of Zinc on microbial biomass carbon, respiration, and community structure in rice soil were studied. Six samples were added concentrations between 50 and 500mg Zn kg-1 soil. The results show that low concentration can promote the growth of microbial biomass carbon in the easily stage of Zn added, but highconcentration can inhibit the growth of microbial biomass carbon. The inhibition is strengthened with increasing concentration and the critical concentration of Zn is about 200mg·kg-1. Analysis of the Biolog data by PCA indicated that microbial community structure 1 had changed, microbial has much more rapid in consuming carbon sources, and have long lasting adverse effects under the stress of Zn contamination. All these changes are correlation with soil binding capacity.