Compound RSA 11β-Hydroxylation of %Curvularia lunata% was studied.The composition of hydroxylation products was analyzed.It was found that the conversion rate with protoplasts was increased conspicuously,the construction and quantity of hydroxylation products was not alteration,but the percentage was changed greatly.The 14α-OH percentage of protoplasts was 3.03 -fold than that of mycelia, the 11α-OH percentage was decreased 60%,and the conversion ratio of 11β-OH was more 1.29-fold than that of the mycelia.It affected the percentage of hydroxylation products when 2 mg/mL polyoxins were added into the mycelia conversion system.