A rapid and simple liquid chromatography method with on-line solid phase extraction was developed and validated for the quantitative determination of cyclophosphamide in rat plasma.The plasma sample was first extracted on an Acclaim？ Polar Advantage II C18 guard column（PA II C18,10 mm×4.6 mm,5 μm）,which was also the on-line Extraction Cartridge SPE column,by washing with 100% H2O for 1 min.The extracted sample was then eluted onto a PA II C18 column（150 mm×4.6 mm,5 μm） and separated by isocratic elution with acetonitrile-water（40：60,v/v）.The mobile phase was run at a flow rate of 1.0 mL/min,and the UV detector was set at 195 nm.Retention time of cyclophosphamide was 4.3 min and the total run-time was 6 min.The linear range of the standard curve was from 1.0 to 200 μg/mL（r2 = 0.9999）,and the limits of quantification and detection were 1.0 μg/mL（RSD10%,n = 5） and 0.3 μg/mL（RSD13%,n = 5）,respectively.Both intra-and inter-day variations were less than 5.6%.The developed method can be used for the therapeutic drug monitoring of cyclophosphamide in the clinic.
N,N-Dimethylamphetamine （DMA） is a methamphetamine （MA） analog and considered as an amphetamine-derived designer drug. In this study, a method of gas chromatography-mass spectrometry （GC-MS） combined with nuclear magnetic resonance spectroscopy （1H and 13C NMR） was developed for the identification of dimethylamphetamine in a suspect exhibit. The results showed that purity of this sample was above 99% by GC-MS area normalization. Therefore, it could be used as a dimethylamphetamine reference substance in drug FTIR analysis, which was successfully applied to identify samples in other similar cases.
