[Objective] The research aimed to study the seed germination and growth of different rape cultivars, including Brassica napus, Brassica juncea and Brassica rapa, treated with kanamycin or hygromycin at different concentrations. [Method] Brassica napus Chun K101, Brassica napus L0935 and Brassica rapa L0926 seeds were cultured on 1/2 MS medium supplemented with different concentrations of antibiotics. The hypocotyl and taproot changes of different rape cultivated were recorded and analyzed. [Result] It was shown that 50 mg/L kanamycin could stimulate rapeseed hypocotyl growth, but obviously restrain taproot development. Hygromycin apparently inhibited the growth of hypocotyl and taproot of all the three cultivars. [Conclusion] Kanamycin was suitable to screen the transformed seed of Brassica juncea. The optimal concentration of hygromycin to screen transgenic rapeseed was 40-60 mg/L.
