Chemical constituents of the whole herb of Rhodiola kirilowii(Regl) Maxim.were investigated.The separation of the constituents was achieved by using a combination of various chromatographic techniques including column chromatography over silica gel,ODS,Sephadex LH-20 and reversed-phase HPLC.Their structures were elucidated by spectroscopic techniques including 1D and 2D NMR spectroscopy.Eleven compounds were obtained and identified as 4-hydroxyphenylethyl-(4'- methoxyphenylethyl)ether(1),1-(2-hydroxy-2-methylbutanoate)-β-D-glucopyranose(2),4-ethoxy-phenylethanol acetate(3),p-hydroxyacetophenone (4),p-hydroxy-benzoic acid ethylester(5),4-hydroxybenzoic acid(6),4-hydroxybenzaldehyde(7),R(-)-mellein (8),stigmasterol(9),4-methoxy-phenylethanol(10),and methylgallate(11).Among them,1 and 2 are new compounds; compounds 3,5,7,8,and 10 were isolated from Rhodiola genus for the first time;and compounds 4,6,9,and 11 were isolated from Rhodiola kirilowii(Regl) Maxim.for the first time.
Phytochemical investigations of Pseudolarix kaempferi Gord led to the isolation of 26 known compounds. They were identified as follows： pseudolaric acid D （1）, pseudolaric acid A （2）, pseudolaric acid B （3）, pseudolaric acid C （4）, oleana- 11,13（18） - dien-3β-O-acetyl （5）, oleana- 9（11）,12 -dien-3β-O-acetyl （6）, oleana- 11,13（18）-dien-3β-hydroxyl （7）, oleana- 9（11）,12 -dien-3β-hydroxyl （8）, celangulatin C （9）, celangulatin E （10）, 17β-tenacigenin B （11）, 11α-O-2-methyl butyryl-12β-O-acetyl tenacigenin B （12）, 11α-O-2-methyl butyryl-12β-O-tigloyl tenacigenin B （13）, β-sitosterol acetate （14）, umbelliferone （15）, 5,7-dihydroxyl coumarin （16）, xanthotoxin （17）, isopimpinellin （18）, formononetin （19）, calycosin （20）, cnidimol B （21）, thymine （22）, 3-furoic acid （23）, 2-furoic acid （24）, vanillic acid （25）, protocatechuic acid （26）. Compounds 5–24 were isolated from P. kaempferi for the first time. And the 1 H and 13 C NMR spectra of compound 1 were completely assigned for the first time.
