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一种在试管中进行番茄组织培养的方法
本发明属于生物技术领域,是涉及一种番茄组织培养方法使用的诱导、分化、生根培养基,利用该培养基番茄实现在试管中开花结果的方法。所述方法包括番茄诱导愈伤组织培养步骤、分化成苗步骤、生根,花芽分化,开花结果步骤;所述方法的整个...
鹿金颖潘毅阚晟沈前华刘敏
The Cloning of Erythropoiesis-related Genes with the Innovated Model System
2002年
Most studies of erythropoiesis have been performed either with the virally transformed MEL cells or with the scarce erythroblasts within unhomogeneous cell populations.MEL cells are the Friend virus transformed mouse erythroleukemia cells and have lost the capacity to respond to erythropoietin.Moreover,the research in the field of erythropoiesis has been severely hampered by the paucity of erythroblasts obtained from chick embryos,human,mouse,rat fetal liver,mouse spleen,or the bone marrow.In our paper,the fetal blood from newly slaughtered pregnant sheep meets the needs of studies as abundant staring biological materials at the nucleotide level and also reflects in vivo proliferative and differential status of erythroblasts.Appling the technique of mRNA differential display with minor modification,we directly compared cDNA fragmerts from the fetal blood erythroblasts and adult pregnant sheep leukocytes by PCR on DNA sequencing gels and revealed two differentially expressing bands.One is a novel gene fragment, which has homology with UV-sensitive cone opsin or rhodopsin by examinating the predicted amino acid sequence.Since the blood cells are sensitive to radiation,it can be safely inferred that this fragment participate in the process of cytocidal effect of the radiation.The other fragment is glucose induced gene /elongation factor 2 3′end noncoding region,which was first cloned from glucose induced bovine aortic smooth muscle cells.Using Northern blot hybridization method,authentic specific expression was confirmed.
阚晟李文雍刘桂生陈清轩
关键词:ERYTHROPOIESISERYTHROBLAST
绵羊(Ovis avies)胚胎红细胞发生相关基因的cDNA克隆
红细胞发生的研究主要依赖于MEL细胞系和分离到的少量的小鼠成红细胞.MEL细胞对促红细胞生成素不敏感而且是Friend病害转化的细胞.来源于鸡胚;人、小鼠、大鼠胎肝;小鼠肾脏或骨髓少量的成红细胞严重地困扰着在分子水平上的...
阚晟
关键词:胎羊
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一种在试管中进行番茄组织培养的方法
本发明属于生物技术领域,是涉及一种番茄组织培养方法使用的诱导、分化、生根培养基,利用该培养基番茄实现在试管中开花结果的方法。所述方法包括番茄诱导愈伤组织培养步骤、分化成苗步骤、生根,花芽分化,开花结果步骤;所述方法的整个...
鹿金颖潘毅阚晟沈前华刘敏
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