[Objective] The aim of this study was to explore the taxonomic status and genetic relationship of ants at molecular level.[Method]Applying cyt b gene as a molecular marker,molecular phylogenetic analysis of 14 ant species of 5 genera(Camponotus,Formica,Polyrhachis,Pheidole and Crematogaster)in Formicidae was conducted.Partial sequences of cyt b gene in 14 ant species were analyzed with software MEGA,Clustal X and PAUP,and phylogenetic trees were constructed by Neighbor-Joining method(NJ)and Maximum-Parsimony method(MP).[Result]NJ tree and MP tree showed that the 14 ant species could be clustered into 5 branches.[Conclusion]The results of molecular phylogenetic analysis coincided with the views of traditional morphological taxonomy.
[Objective] This study aimed to explore the conditions of transformation of maize by microprojectile bombardment. [Method] Immature embryo-derived callus of maize inbred line 7239 was used as explants to study the effects of shoot distance, helium pressure, vacuum and bombardment frequency on the transformation efficien- cy in the particle bombardment system of maize. [Result] Considering the transfor- mation efficiency, particle bombardment with 100 μg/P of golden particles, at a shoot distance of 9 cm from the target cells, under helium pressure of 1 350 psi and vac- uum 25 inHg, and bombarding twice could achieve relatively ideal results. After se- lection on media supplemented with different concentration of hygromycin, some re- generated plants were obtained. The results of PCR and Southern blotting analysis demonstrated that the NPR1 gene had been integrated into the genome of trans- genic maize plants, with an average transformation efficiency of 1.76%. [Conclusion] The study laid the foundation for the cultivation and breeding of excellent resistant varieties of maize.
