The callus is initiated in three media (B5,MS,White) from the buds,leaves and stem segments of Buddleja officinalis.The results shows that the inducing frequencies of tender leaves of buddleja offcinalis on media are as high as 100%.No significant differences between tender leaves and buds is observed,as to the frequency.The frequency was decreased as the leaf became older.The medium with 6-BA 0.5mg/L and NAA 1.0mg/L is selected as the best medium for callus culture.However,B5 is more suitable for calli and cells to grow and divide than MS and white.The growth rate of leaves is faster than buds and stem segments.The Medium with 6-BA 0.5mg/L and NAA 1.0mg/L is applicable to increase the cell growth.
