口腔疾病是儿童较为常见的病症,该病症近年来发病率呈现出明显的增长趋势,严重影响儿童身心健康,不利于儿童健康成长,降低生活质量。临床治疗中,新型生物陶瓷材料逐渐被广泛的应用,有效提升整体治疗效果,并降低治疗后不良反应,因而本文针对儿童口腔治疗恒牙和乳牙中应用新型生物陶瓷材料iRoot BP Plus的临床实践开展探索,旨在为其临床应用实践奠定良好的基础。
[Objective] This study aimed to establish a real-time PCR method for de- tecting stx2 gene in Shiga toxin-producing E. coli （STEC）. [Method] According to the known STEC stx2 gene sequences published in GenBank, PCR primers and probes were designed based on the conserved region to construct recombinant plasmid as a positive template, thus optimizing the reaction conditions and establishing the real- time PCR method. [Result] A standard curve was established based on the opti- mized real-time PCR system, indicting a good linear correlation between the initial template concentration and Ct value, with the correlation coefficient F^e of above 0.995. The established method had a good specificity, without non-specific amplifica- tion for 10 non-STEC intestinal bacterial strains; the detection limit of initial template was 1.0x102 copies/μI, indicating a high sensitivity; furthermore, the coefficients of variation within and among batches were lower than 1% and 5% respectively, sug- gesting a good repeatability. [Conclusion] In this study, a real-time PCR method was successfully established for detecting STEC stx2 gene, which provided technical means for rapid detection of STEC in samples.
